Studi Gen Parp-1 Ekson 23 pada Testis Mencit Setelah Induksi Formalin: Tidak Terdeteksi Mutasi
Abstract
Penelitian  ini  bertujuan  untuk  mengetahui pengaruh induksi formalin terhadap mutasi pada gen PARP-1 ekson 23. Penelitian ini menggunakan hewan coba mencit (Mus musculus) Balb-c jantan berumur 3 bulan, berat 20-30 g, yang dibagi menjadi 3 kelompok, yaitu kelompok kontrol tanpa paparan formalin (KB), kelompok formalin dosis 2 mg/kg BB (F2), dan kelompok formalin dosis 25 mg/kg BB (F25). Sediaan formalin dalam bentuk cairan diberikan secara peroral menggunakan sonde selama 60 hari. DNA diisolasi dari organ testis dilakukan amplifikasi menggunakan primer forward (5’-GTTGTTTGTGGTT TGTCCTA-3’) dan reverse (5’-TTTTTAGCTCAAAATAAATGCTTAA-3’). Hasil sekuensing menunjukkan tidak ada perubahan basa nukleotida (mutasi) gen PARP-1 ekson 23 pada testis mencit (Mus musculus) Balb-c setelah induksi formalin.References
Cahyadi, W. 2006. Analisis dan Aspek Kesehatan: Bahan Tambahan Pangan. Bumi Aksara. Jakarta.
Norliana, S., Abdulamir A.S., Abu Bakar F., & Salleh A.B. 2009. The Health Risk of Formaldehyde to Human Beings. American Journal of Pharmacology and Toxicology, 4 (3): 98-106.
Allen, R.G. & Tressini M. 2000. Oxidative Stress and Gene Regulation. Free Radical Biology Medicine, 28: 463-499.
Widyarti, S. & Wibi R. 2009. Laporan Penelitian Hibah Penelitian Strategis Nasional: Potensi Formalin dalam Meningkatkan Kerentanan Mutasi Gen Parp-1 dan Suseptibilitas terhadap Bahan Karsinogenik. Universitas Brawijaya. Malang.
Kim, M.Y., Zhang T., & Kraus W.L. 2005. Poly(ADP-ribosyl)ation by PARP-1: ‘PAR-laying’ NAD+ into a Nuclear Signal. Genes Development, 19: 1951-1967.
Yelamos, J., Jordi F., Laura L., Coral A., & Juan M.C. 2011. Review Article PARP-1 and PARP-2: New Players in Tumour Development. American Journal of Cancer Research, 1 (3): 328-346.
Golalipour, M.J., Azarhoush R., Ghafari S., Gharravi A.M., Fazeli S.A., & Davarian A. 2007. Formaldehyde Exposure Induces Histopathological and Morphometric Changes in The Rat Testis. Folia Morphology, 66 (3): 167-171.
Imron, M.T.A. 2010. Studi Paparan Formalin terhadap Struktur Histologi Testis Mencit (Mus musculus) BALB/c. Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya. Malang. Skripsi.
Ausubel, F.M., Roger B., Robert E.K., David D.M., Seidman J.G., John A.S., & Kevin S. 2003. Current Protocols in Molecular Biology. John Wiley & Sons, Inc. USA.
Zhou, D.X., Qiu S.D., Wang Z.Y., & Zhang J. 2005. The Protective Effect of Vitamin E Against Oxidative Damage Caused by Formaldehyde in the Testes of Adult Rats. Asian Journal of Andrology, 8: 584-588.
Alano, C.C., Garnier P., Ying W., Higashi Y., Kauppinen T.M., Swanson R.A. 2010. NAD+ Depletion is Necessary and Sufficient for Poly(ADP-ribose) Polymerase-1 Mediated Neuronal Death. Journal Neurosci, 30: 2967-2978.
Meyer-Ficca, M.L., Lonchar J., Credidio C., Ihara M., Li Y., Wang Z.Q., & Meyer R.G. 2009. Disruption of Poly(ADP-Ribose) Homeostasis Affects Spermiogenesis and Sperm Chromatin Integrity in Mice. Biology of Reproduction, 81: 46-55.
El-Domyati, M.M.M.D., Abo B.M.A.D.M.D., Manal T.B.M.D., Hasan M.E.F.M.D., Jiasen X., & Denny S. 2009. Deoxyribonucleic Acid Repair and Apoptosis in Testicular Germ Cells of Aging Fertile Men: The Role of The Poly(adenosine diphosphate-ribosyl)ation Pathway. Elsevier, 91 (5): 2221-2229.
Corebima, A.D. 2000. Genetika Mutasi dan Rekombinasi. Handout Perkuliahan. Universitas Negeri Malang. Malang.
Myers, J.A., Mall J., Doolas A., Jakate S.M., & Saclarides T.J. 1997. Absorption Kinetics of Rectal Formalin Instillation. World Journal of Surgery, 21: 886-889.
Eells, J.T., Mc Martin, Black K. 1981. Formaldehyde Poisoning: Rapid Metabolism to Formic Acid. JAMA, 246:1237-1238.
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