EKSPRESI PROTEIN P53 PADA SEL TIG-3 SETELAH PERLAKUAN SINAR UV DAN EKSTRAK BIJI JUWET (Syzygium cumini)
Abstract
Tubuh terlindungi dari lingkungan luar karena adanya kulit. Adanya paparan lingkungan luar, seperti sinar UV dapat mempengaruhi sel pada kulit. Dalam jangka pendek dapat menyebabkan kulit terbakar serta dalam jangka panjang menyebabkan kanker atau penuaan dini. Salah satu cara yang biasa digunakan untuk melindungi sel kulit dari paparan sinar UV adalah dengan penggunaan antioksidan. Jambolan (S. cumini) merupakan salah satu tumbuhan yang memiliki kandungan antioksidan yang tinggi. Protein p53 merupakan salah satu protein yang bertugas dalam melindungi DNA dari kerusakan yang ditimbulkan setelah sel kulit terpapar sinar ultraviolet (UV). Penelitian ini bertujuan untuk melihat ekspresi protein p53 pada sel fibroblast TIG-3 setelah dipapar sinar UV serta pemberian ekstrak biji juwet. Sel dikultur dan dipapar sinar UV 30mJ/cm2 lalu diberi ekstrak biji juwet dengan dosis 0,1% dan 10%. Pemberian ekstrak biji juwet mampu meningkatkan ekspresi protein p53 pada sel TIG-3.
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Kata kunci: ellagitannin, fibroblas, jambolan, p53, UV.
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