Prevalensi dan Identifikasi Berdasarkan Sekuen ITS Kapang Patogen Batang Tanaman Apel Var. Anna (Malus sylvestris (L.) Mill.) di Kota Batu
Abstract
ABSTRAK
Penurunan jumlah pohon apel produktif mengakibatkan rendahnya daya saing apel di dalam negeri. Berkurangnya jumlah tanaman apel produktif salah satunya disebabkan oleh kapang patogen. Penelitian ini bertujuan untuk mengidentifikasi spesies kapang patogen yang menyerang batang tanaman apel berdasarkan sekuen ITS. Sampel diambil dari perkebunan Gabes, Dusun Junggo, Desa Tulungrejo Kecamatan Bumiaji, Kota Batu. Kapang diisolasi dari lima batang bergejala penyakit dan dimurnikan, diuji patogenisitas terhadap bibit tanaman apel, isolasi DNA, dan identifikasi molekular. Berdasarkan hasil penelitian gejala AN3 banyak ditemui pada perkebunan organik dengan prevalensi 59,6 , sedangkan gejala AN5 merupakan gejala penyakit yang paling sedikit dengan prevalensi 1,69. Isolat AN5.2 teridentifikasi sebagai Neofusicoccum parvum yang menyebabkan penyakit busuk kering dan berakibat kematian pada batang, sedangkan isolat AN3.2 teridentifikasi sebagai Aureobasidium pullulans.
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Kata kunci: Aureobasidium pullulans, identifikasi molekular, kapang patogen, Malus sylvestris Mill., Neofusicoccum parvum
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