Bioactivity of Proplis to the CD4 + and CD8+ T cells Producing IFN-γ Cytokines in BALB / C Mice
Abstract
ABSTRACT
Propolis (bee glue) is a natural resinous product of honey bees which collected from exudates and plant buds, rich in biochemicals constituents including mostly flavonoids, phenols and various acids bond. These compound are believed to be responsible as immunomodulatory agents. The study aims to determine the immunomodulatory activity of ethanolic extract of propolis to the CD4+ and CD8+ T cells producing IFN-γ cytokines and analyze the differences immune responses between control and treatment group by in vivo. Stages include animal acclimation for ± 1 week, preparation of Ethanolic Extracts of Propolis / EEP, Oral Administration with doses levels of 0 mg / kg BW; 50 mg / kg BW (DI); 100 mg / kg BW( DII); 200 mg / kg BW (DIII) for 2 weeks, isolation of lymphocyte cells from spleen, flowcytometry analysis to asses cell number and surface molecule expression. Data was analyzed using Kruskal Wallis Test with α = 0,05 and followed by Mann Whitney Test by SPSS 16.0 for windows with complete randomized design. The results showed that a dose of 50 mg / kg BW was increases the relative number of CD8+ T cells producing IFN-γ cytokines significantly (p <0.05) compared with controls. However, at the same dose the relative number of CD4+ T cells producing IFN-γ cytokines was decreased significantly (p<0,05). Based on this case, its dose supposedly that the ethanolic extract of propolis play role in maintaining the balance or homeostatic of IFN-γ cytokines production by T cell subsets. Dose of 100 mg/kg BW and 200 mg / kg BW could decrease the relative number of activated CD4+ T cells producing IFN-γ cytokine significantly compared to controls.
Keywords: CD4+ T cells, CD8+ T cells, ethanolic extract of propolis, IFN-γ cytokines, in vivo
References
REFERENCES
Bankova, V., Castro, S.L & Marcucci, M.C. 2000. Propolis:recent advances in chemistry and plant origin. Apidologie.3:3–15.
Burdock, G.A. 1998. Review of the biological properties and toxicity of bee propolis (propolis). Food and Chemical Toxicology.36:347–363.
Siregar, H.C.N., Asnanth M.F & Yuke, O. 2011. Propolis Madu Multikhasiat. Penebar Swadaya. Jakarta.
Girgin, G., Baydar, T., Ledochowski, M., Schennach, H., Bolukbasi, D.N., Sorkun, K., Salih, B., Sahin, G & Fuchs, D. 2009. Immunomodulatory Effect of Turkish Propolis :Changes in neopterin Release and Tryptophan Degradation. Immunobiology. 214 (2):129-34.
Nazir, N. 2013. Imunomodulatory Activity of Isoflavones Isolated from Iris Kashmiriana: Effect on T-Lymphocyte Proliferation and Cytokine Production in Balb/C Mice. Journal of Biomedicine & Preventive Nutrition. 3:151–157.
Challem, J. 2004. Tuberculosis, Medical Journals Document Value of Bee Propolis, Honey and Royal Jelly. The Nutrition Reporter.
Park, J.H., J.K.Lee, H.S., Kim, S.T., Chung, J.H., Eom, K.A.,Kim, S.J., Chung, S.U. Paik & H.Y. Oh. 2004. Immunomodulatory effect of caffeic acid phenethyl ester in Balb/c mice. Intl. Immunopharmacol. 4: 429-436.
Fatahinia, M., Khosravi, A.R & Shokri, H. 2012. Propolis efficacy on TNF-α, IFN-γ and IL-2 cytokine production in old mice with and without systemic candidiasis. Journal de Mycologie Medicale. 22,237-242.
Wahab, Shadma & Hussain, Arshad. 2013. Cytokines As Targets For Immunomodulation. International Journal of Pharmacy and Pharmaceutical Sciences. Vol 5, Suppl 3.
Baratawidjaja, K.G dan Rengganis, Iris. 2010. Imunologi Dasar Edisi Ke-9. Balai Penerbit, Fakultas Kedokteran Universitas Indonesia.
Robinson, T. 1995. Kandungan Organik Tumbuhan Tinggi. ITB. Bandung.
Ansorge, S., Reinhold, D., & Lendeckel U. 2003. Propolis and some of its constituents down-regulate DNA synthesis and inflammatory cytokine production but induce TGF-beta production of human immune cells. Zeitschrift fur Naturforschung. 58c:580—9.
Rifa’i, M., Z. Shi, S.Y. Zhang, Y.H. Lee, H. Shiku, K. Isobe, and H. Suzuki. 2008. CD8+CD12+ regulatory T cells recognize activated T cells via conventional MHC class I–αβTCR interaction and become IL-10-producing active regulatory cells. International immunology. 20. 937-947.
Cruse, J.M dan Lewis, R.E. 2010. Atlas of Immunology, Second Edition. CRC Press. United States.
Santamaria, Pere. 2002. Cytokines and Chemokines in Autoimmune Disease. Advances in Experimental Medicine and Biology. Vol 520.
Nelson, Brad.H. 2004. IL-2, Regulatory T Cells and Tolerance. J Immunol.172:3983-3988.
Rifa’i,M., Y Kawamoto, I Nakashima, H Suzuki . 2004. Essential roles of CD8+CD122+ regulatory T cells in the maintenance of T cell homeostasis. The Journal of experimental medicine 200(9), 1123-1134.
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