Characterization and Antibacterial Activity of Endophytic Bacteria from Flesh Fruit of the Arecaceae Family Against Antibiotic Resistant Bacteria <i>Escherichia coli</i>
DOI:
https://doi.org/10.21776/ub.biotropika.2021.009.02.11Keywords:
Endophytic bacteria, Arecaceae, antibiotic resistant, Escherichia coliAbstract
Bacteria can symbiotic mutualism and synergize with plants in the tissue and do not cause harm to plants. On the other hand, bacteria resistance provides an opportunity to search for antibacterials from natural material without threatening plant biodiversity. The purpose of this study was to characterize and test the antibacterial activity of endophytic bacteria from the fruit of the Arecaceae family against antibiotic-resistant bacteria Escherichia coli. The method used was direct culture on the growth of media in the laboratory by first sterilizing the surface of the flesh of the samples, such as Aren (Arenga pinnata), Coconut (Cocos nucifera), and Sago (Metroxylon sagu). The pure isolates obtained were characterized according to Bergeys Manual for Identification by performing Gram stain, catalase, and coagulase test. The results obtained were five isolates with codes A1, A2, K, S1, and S2 with variation in morphology colonies. Gram staining, catalase, and coagulase verification of the five isolates were groups of Grampositive cocci, catalase-positive, and coagulase-negative bacteria. Based on tests carried out on the characters of the endophytic bacteria, it was obtained a close relationship with the genus Staphylococcus. The results of the inhibitory activity test showed that Staphylococcus sp. K with the highest criteria of 43.26 mm and Staphylococcus sp. A1 with the lowest inhibition criteria with a value of 24.73 mm. Both are categorized as very strong inhibition criteria.References
Dinas Kesehatan Aceh (2017) Profil Kesehatan Aceh Tahun 2016. www.dinkes.acehprov.go.id. Diakses 15 Juli 2019.
Dinas Kesehatan Aceh (2018) Profil Kesehatan Aceh Tahun 2017. www.dinkes.acehprov.go.id. Diakses 15 Juli 2019.
Kementrian Kesehatan Republik Indonesia. (2019) Data Dan Informasi Profil kesehatan Indonesia 2018. http://www.depkes.go.id/index.php?txtKeyword=Data+Dan+Informasi+Profil+kesehatan+Indonesia+2018&act=searchaction&pgnumber=0&charindex=&stru&fullcontent=&CALL=1&C1=1&C2=1&C3=1&C4=1&C5=1. Diakses 18 Juli 2019.
Roswita C (2018) Pemanfaatan tumbuhan palem – paleman (Arecaceae) sebagai obat tradisional oleh masyarakat Aceh di Kecamatan Gandapura Kabupaten Bireuen. Biosains 4: 32-38.
Bhore S (2010) Screening of endophytic bacteria isolated from leaves of Sambung Nyawa [Gynura procumbens (Lour.) Merr.] for cytokinin-like compounds. Bioinformation 5(5): 191-197.
Tadych M, James FW (2018) Endophytic Microbes. Chapter. Rutgers University, New Brunswick, NJ, USA.
Kusumawati DE, Fachriyan HP, Maria B (2014). Aktivitas antibakteri isolat bakteri endofit dari tanaman miana (Coleus scutellariodes L. Benth.) terhadap Staphylococcus aureus dan Escherichia coli. Current Biochemistry 1(1): 45-50.
Labrador KL, Lustica ELT, Novero AU (2014). Isolation and characterization of bacterial endophytes associated with sago palm (Metroxylon sagu Rottb.) in tissue culture. Asian J. of Microbiol. Biotechnology Environment Science 16: 877-886.
Jauhari LT (2010). Seleksi dan Identifikasi apang Endofit Penghasil Antimikroba Penghambat Pertumbuhan Mikroba Patogen. [Skripsi]. Departemen Biologi Fakultas Sains dan Teknologi. UIN. Jakarta.
Aqlinia M, Pujiyanto S, Wijanarka (2020) Isolasi bakteri endofit bangle (Zingiber cassumunar Roxb.) dan uji antibakteri supernatan crude metabolit sekunder isolat potensial terhadap Staphylococcus aureus. J. Akad. Biol. 9: 23–31.
Karl HS, Julia AB (2015) Bergey's Manual of Systematics of Archaea and Bacteria. John Wiley & Sons, Inc.
Strobel GA, Daisy B (2003) Bioprocessing for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews 67: 491-502.
Cristina A, Varghese C, Subhash JB (2013) Endophytic bacteria as a source of novel antibiotics: An overview. Pharmacogn Rev. 7(13): 11–16.
Trivedi PC, Pandey, Bhadauria (2010) Text book of microbiology. Aavishkar Publishers. India
Seo WT, Lim WJ, Kim EJ, Yun HD, Lee YH, Cho KM (2010) Endophytic bacterial diversity in the Young Radish and their antimicrobial activity against pathogens.Korean Soc. Appl. Biol.Chem. 53(4): 493-503.
Koomnok C, Teaumroong N, Rerkasem B, Lumyong S (2007) Diazotroph endophytic bacteria in cultivated and wild rice in Thailand. Science Asia 33: 429-435.
Tan RX, Zou WX (2001) Endophytes: a rich source of functional metabolites. Nat. Prod. Rep 18: 448-459.
Sumampouw M, Bara R, Awaloei H, Posangi J (2014) Uji efek antibakteri jamur endofit akar bakau Rhizophora stylosa terhadap bakteri Staphylococcus aureus dan Escherichia coli. Jurnal e-Biomedik 2(1).
Susanto D, Sudrajat, Ruga R (2012) Studi kandungan bahan aktif tumbuhan meranti merah (Shorea leprosula Miq) sebagai sumber senyawa antibakteri. Mulawarmnan Scientifie 11(2): 181-190.
Brooks G, Janet S, Butel L, Nicholas O (2005) Mikrobilologi Kedokteran. Jakarta, EGC.
Prescott LM, Harley JP (2002) Laboratory exercises in microbiology. McGrawHill Science.
Strobel GA (2002) Microbial gifts from rain forests. Canadian Journal of Plant Pathology, 24(1): 4-20.
Sunariasih, Linda NP, Suada IK, Suniti NW (2014). Identifikasi jamur endofit dari biji padi dan uji daya hambatnya terhadap Pyricularia oryzae Cav. Denpasar. E-jurnal ageoteknologi tropika 3(2): 51-60.
Kusumawati DE, Fachriyan HP, Maria B (2014). Aktivitas antibakteri isolat bakteri endofit dari tanaman miana (Coleus scutellariodes [L.] Benth.) terhadap Staphylococcus aureus dan Escherichia coli. Curr. Biochemistry 1: 45-50.
Baquero F, Blazquez J (1997) Evolution of antibiotic resistance. Trends Ecol Evol 12(12): 482-487.
Lipsitch M, Samore MH (2002) Antimicrobial use and antimicrobial resistance: a population perspective. Emerg Infect Dis 8(4): 347-354.
Thomas CM, Nielsen KM (2005) Mechanisms of, and barriers to, horizontal gene transfer between bacteria. Nat Rev Microbiol 3(9): 711-721.
Furuya EY, Lowy FD (2006) Antimicrobialresistant bacteria in the community setting. Nat. Rev. Microbiol 4(1): 36-45.
Hughes VM, Datta N (1983) Conjugative plasmids in bacteria of the ‘pre-antibiotic’ era. Nature (302): 725–726.
Kusumaningsih A (2012) Faktor pemicu foodborne disease asal ternak. Wartazoa 22(3): 107-112.
Cowan ST, Steel’s (1993) Cowan and Steel’s manual for the identification of medical bacteria. New York, Cambridge University Press.
Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994). Bergey’s manual of determinative bacteriology. 9th ed. Baltimore: Lippincott Williams & Wilkins.
Suhandono S, Meirina KK, Pingkan A (2016). Isolation and molecular identification of endophytic bacteria from rambutan fruits (Nephelium lappaceum L.) cultivar Binjai. Hayati Journal of Bioscience 23(1): 39-44.
Sepriana C, Eti S (2020) Identifikasi dan uji daya hambat isolat bakteri endofit bunga tanaman cengkeh (Syzygium aromaticum L.) terhadap bakteri patogen. Penelitian Pendidikan IPA 6(1): 101-106.
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