Oral Administration of The Hypercholesterol Rat Feed Formula to Making The Animal Dyslipidemia Model on Sprague Dawley Rats

Authors

  • Fatchiyah Fatchiyah 1) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya, Indonesia 2) Research Center of Smart Molecule of Natural Genetics Resources (SMONAGENES), Universitas Brawijaya, Indonesia
  • Eko Suyanto 1) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya, Indonesia 2) Research Center of Smart Molecule of Natural Genetics Resources (SMONAGENES), Universitas Brawijaya, Indonesia
  • Rista Nikmatu Rohmah 1) Research Center of Smart Molecule of Natural Genetics Resources (SMONAGENES), Universitas Brawijaya, Indonesia 2) Institute Biosains, Universitas Brawijaya, Malang
  • Lidwina Faraline Triprisila
  • Hazna Noor Meidinna 1) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya, Indonesia 2) Research Center of Smart Molecule of Natural Genetics Resources (SMONAGENES), Universitas Brawijaya, Indonesia
  • Dewi Ratih Tirto Sari 1) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya, Indonesia 2) Research Center of Smart Molecule of Natural Genetics Resources (SMONAGENES), Universitas Brawijaya, Indonesia
  • Iva Himmatul Aliyah 1) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya, Indonesia 2) Research Center of Smart Molecule of Natural Genetics Resources (SMONAGENES), Universitas Brawijaya, Indonesia

DOI:

https://doi.org/10.21776/ub.biotropika.2021.009.02.08

Keywords:

dyslipidemia, high fat diet goat, experimental animals, cholesterol, SpragueDawley rats

Abstract

The aim of this study was to make animal dyslipidemia models in Sprague Dawley strains induced by a high fat goat diet formula as hypercholesterol feed for two months. The experimental animal used in this study was 30 male rats (Rattus norvegicus strain Sprague Dawley) with an age of 2-3 months with an average body weight of 150g. Animal models are divided into two groups consisting of a control group without additional diet and dyslipidemia group given food consumption goat hypercholesterolemia with high-fat diet formula orally every day for two months. Physiological characteristics of dyslipidemia SD rats had higher body weight, increased food consumption and fecal weight, and decreased water intake and urine volume than the control group. Total cholesterol, triglyceride, and LDL-cholesterol levels increased, while HDL-cholesterol levels did not change in the dyslipidemia rats group compared to the control group. The conclusion of this study indicated that the hypercholesterol diet formula with a high composition of goat fat was successfully induced the SD rats to become dyslipidemia model rat with specific hypercholerol characteristics.

Author Biography

Lidwina Faraline Triprisila

1) Research Center of Smart Molecule of Natural Genetics Resources (SMONAGENES), Universitas Brawijaya, Indonesia
2) Institute Biosains, Universitas Brawijaya, Malang

References

Andersen ML, Winter LMF. Animal models in biological and biomedical research – experimental and ethical concerns. Anais da Academia Brasileira de Ciencias 2019;91:1–14. http://dx.doi.org/10.1590/0001-3765201720170238

Joost H-G, Al-Hasani H, Schurmann A. Animal Models in Diabetes Research. Humana Press, 2012 doi:https://doi.org/10.1007/978-1-62703-068-7.

Maurer KJ, Quimby FW. chapter 34 - Animal Models in Biomedical Research. Third Edition. Elsevier Inc., 2015 doi:10.1016/B978-0-12-409527-4.00034-1.

Otto GM, Franklin CL, Clifford CB. Chapter 4 - Biology and Diseases of Rats. 2015 doi:10.1016/B978-0-12-409527-4.00004-3.

Simbolon IS, Lubis TM, Adam M. Persentase Spermatozoa Hidup Pada Tikus Wistar Dan Sprague-Dawley. Jurnal Medika Veterinaria 2013;7(2). doi:10.21157/j.med.vet..v7i2.2935.

Ohta T, Toriniwa Y, Ryumon N, Inaba N, Hirao T, Yamanaka S, Maeno T, Sakakibara W, Chiba K, Nakamura A, Miyajima K, Fatchiyah F, and Yamada T. (2017) Maternal high-fat diet promotes onset of diabetes in rat offspring. Animal Science 88(1): 149–155. https://doi.org/10.1111/asj.12606

Fatchiyah F (2015) Pendaftaran paten No. P00201507845 berjudul “Formula diet hiperkolesterol dengan kandungan tinggi lemak kambingâ€: P00201507845. DJRI, Kemenhunkam-Ham RI

Margarita Y, Princen P, Andi A, Rumawas ME, Kidarsa VB, Sutrisna B. Kadar Kolesterol Total dan Tekanan Darah Orang Dewasa Indonesia. Kesmas: National Public Health Journal 2013;8(2):79.

Brito-Casillas Y, Melián C, Wägner AM. Estudio de la patogénesis y tratamiento de la diabetes mellitus a través de modelos animales. Endocrinologia y Nutricion 2016;63(7):345–353. http://dx.doi.org/10.1016/j.endonu.2016.03.011

Chatzistefanidis D, Markoula S, Vartholomatos G, Milionis H, Miltiadous G, Georgiou I, et al. First Detection of Hypercholesterolemia Causing ApoB-100 R3527Q Mutation in a Family in Greece. Journal of Genetic Syndromes & Gene Therapy 2013;04(06):4–7. doi:10.4172/2157-7412.1000155

Chou J, Ito T, Bishop D, Otsuka M, Ben-Nissan B, Milthorpe B. Controlled Release of Simvastatin from Biomimetic β-TCP Drug Delivery System. PLoS ONE 2013;8(1):2–7.

van Lennep HWOR, Westerveld HT, Zwinderman AH, van Lennep JER, Slot HB, Erkelens DW, et al. Differential effect of female gender on coronary artery disease and peripheral artery disease. Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation 2002;10(12):500–505.

JoAnne, Silbert-Flagg, Elizabeth DS. Pediatric Nurse Practitioner Certification Review. The Nurse Practitioner 2001;26(12):61.

Kattoor AJ, Mehta JL. Alternate day versus daily atorvastatin in low-density lipoprotein cholesterol reduction: A meta-analysis. Current Research: Cardiology 2016;3(3):71–74.

Downloads

Published

2021-08-31

Issue

Vol. 9 No. 2 (2021)

Section

Articles

License

Copyright and Attribution:

Articles in Biotropika: Journal of Tropical Biology are under Creative Commons Attribution-NonCommercial (CC-BY-NC) copyright. The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to the automatic transfer of the copyright to the publisher.

Journal of Biotropika: Journal of Tropical Biology is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0).

Permissions:

Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from one of the authors.

Ethical matters:

Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the author's own must have had the correct authorization for this exportation.