Skrining dan Identifikasi Bakteri Pektinolitik Endosimbion dalam Sistem Pencernaan Serangga Penggerek Kopi (Hypothenemus hampei Ferr)
DOI:
https://doi.org/10.21776/ub.biotropika.2019.007.02.1Keywords:
skrining, pektinolotik, H. hampeiAbstract
Serangga Hypothenemus hampei memiliki kemampuan untuk menggerek biji kopi, dan digunakannya sebagai sumber makanan, tempat hidup dan siklus hidup. Aktivitas hidup dari H. hampei menyebabkan rontoknya buah kopi dan penurunan kualitas serta kuantitas buah kopi hingga 40%. Penelitian ini bertujuan untuk mendapatkan isolat bakteri pektinolitik yang memiliki toleransi tinggi terhadap lingkungan khususnya kafein, tanin dan polifenol. Prosedur penelitian terdiri dari tahap pembuatan media skrining bakteri pektinase, isolasi bakteri endosimbion dan identifikasi spesies serta fisiologi bakteri. Berdasarkan hasil identifikasi dan skrining bakteri pada sistem pencernaan H. hampei didapatkan 15 isolat bakteri pektinolitik endosimbion. Hasil identifikasi tiga isolat terpilih diketahui bahwa isolat 2B termasuk golongan Enterobacter sp. atau Actinobacillus sp., isolat 31B masuk ke dalam Micrococcus sp. dan isolat 42B termasuk Chromobacterium sp. Penelitian ini berguna sebagai kajian untuk strategi penanganan hama serangga H. hampei dengan menghambat pertumbuhan bakteri pektinolitik.References
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