Pengaruh Ekstrak Ethanol Kemangi (Ocimum canum Sims.) terhadap Struktur Histologi Testis Mencit (Mus musculus) Jantan
DOI:
https://doi.org/10.21776/ub.biotropika.2017.005.01.4Abstract
Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak ethanol kemangi (Ocimum canum Sims.) terhadap struktur histologi testis mencit jantan (Mus musculus). Penelitian ini menggunakan 9 ekor hewan coba mencit strain Balb/c jantan berumur 3 bulan, berat badan 20 - 30 g. Hewan coba dibagi menjadi tiga (3) kelompok kontrol tanpa pemberian ekstrak kemangi (P0), kelompok yang diberi ekstrak kemangi dengan dosis 100 mg/kgBB (P1) dan 200 mg/kgBB (P2). Ekstrak kemangi diberikan setiap hari secara oral selama 35 hari. Parameter yang diamati adalah diameter tubulus seminiferus, tebal epitel germinal, jumlah lapisan germinal dan jumlah sel spermatogenik meliputi spermatogonia, spermatosit dan spermatid. Data yang diperoleh dianalisis dengan One-way ANOVA (p ≤ 0,05). Jika menunjukkan perbedaan yang bermakna, maka dilanjutkan dengan uji Least Significant Difference (LSD). Hasil penelitian menunjukkan bahwa pemberian ekstrak kemangi dosis 200 mg/kgBB meningkatkan diameter tubulus seminiferus, tebal epitel germinal, dan jumlah sel spermatidReferences
Huhtaniemi, I.T. 2009. Endocrine Regulation Of Male Reproduction. University of Turku. Finland.
Droge W. 2002. Free radicals in the physiological control of cell function. Physiol Rev. 82:47-95.
Abuja, P.M., Albertini, R. 2001. Methods for monitoring oxidative stress, lipid peroxidation and oxidation resistance of lipoproteins. Clin Chim Acta. 306 : 1-17.
Hadi, R.S. 2011. Apoptosis pada Sperma Sebagai Pertanda Adanya Gangguan Kesuburan Pria. Majalah Kesehatan PharmaMedika. Vol 3 (2).
Aluko, B.T., O.I. Oleyede., A.J. Afolayan. 2012. Full Length Research Paper Phytochemical and nutrient compositions of the leaves of Ocimum canum Sims. African Journal of Biotechnology. Vol. 11 (63) hal: 12697-12701.
Shadia, S. 2007. Chemical Composition of Ocimum Americanum Essential Oil and Its Biological Effects Against, Agrotis ipsilon, (Lepidoptera: Noctuidae). Research Journal of Agriculture and Biological Sciences. Vol 3(6): 740.
Murod, Auva Marwah. 2014. Uji Aktivitas Ekstrak Air Herba Kemangi (Ocimum americanum L.) terhadap Kualitas Sperma dan Densitas Sel Spermatogenesis Tikus Sprague – Dawley Jantan secara in vivo. Program Studi Farmasi FakultasKedokteran dan Kesehatan, UIN Syarif Hidayatullah. Jakarta. Skripsi
Cheng, C. Yan. 2008. Molecular Mechanisms in Spermatogenesis. Center for Biomedical Research The Population Council New York. USA
Hayati, Alfiah., B. Yunaida., I.B.R. Pidada., W. Darmanto., D. Winarni. 2004. Efek 2-Methoxyethanol terhadap Struktur Histologi Testis Mencit (Mus musculus). Berkas Penelelitian Hayati. Vol (10): 7-12.
Chauhan, N.S and Dixit V.K. 2008. Original Article Spermatogenic Activity of Rhizomes of Curculigo orchioides Gaertn in Male Rat. International Journal of Applied Research in Natural Products. Vol. 1(2): 26-31.
Somoyani, N. Ketut. 2011. Pemberian Astaxanthin Oral Mencegah Gangguan Spermatogenesis Mencit (Mus musculus) yang Diinduksi Pelatihan Fisik Berlebih. Program Magister Program Studi Ilmu Biomedik Program Pascasarjana Universitas Udayana. Denpasar. Tesis.
Widhyari, S.D., A. Esfandiari., A, Wijaya., R. Wulandari., S. Widodo., L. Maylina. 2015. Tinjauan Penambahan Mineral Zn dalam Pakan Terhadap Kualitas Spermatozoa pada Sapi Frisian holstein Jantan. Jurnal Ilmu Pertanian Indonesia (JIPI). Vol. 20 (1): 72 77.
Cheah, Yunsang., Wanxi. Yang. 2011. Functions of essential nutrition for high quality spermatogenesis. Bioscience and Biotechnology. Vol 2 (182-197).
Conquer, J.A., Martin, J.B., Tummon, I., Watson, L., Tekpetey, F. .2000. Effect of DHA Supplementation on DHA Status and Sperm Motility in Asthenozoospermic Males. Lipids. Vol 35: 149-154
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