EXPLORATION NON-SYMBIOTIC NITROGEN FIXING BACTERIA PRODUCED IAA (INDOLE ACETIC ACID) AND PHOSPHATE SOLUBILIZATION FROM APPLE’S TREE RHIZOSPHERE IN BATU, EAST JAVA
Abstract
Biofertilizer is fertilizer contain microbes that help provide available nutriens for plants. Non symbiotic nitrogen fixing bacteria has been widely utilized as a biofertilizer agent. The objective of this research was to explore bacteria have ability in fixing nitrogen, producing IAA (indole acetic acid) and solubilizing phosphate from rhizosfer of Apple tree in Batu City. East Java. Isolation of soil sample from Apple tree rhizosphere was carried out using serial dilution. Nitrogen fixation ability was assayed qualitatively using nitrogen free bromothymol blue (Nfb) medium enriched with tryptophan. Quantitative measurement of Nitrogen fixation was done by Visocolor ammonium alpha detection kit. IAA production was observed in Luria Bertani medium enriched with tryptophan and Salkowski reagent. Detection of phosphate solubilization was done using Pikovskaya agar and Mo-blue reagent. Four isolates were obtained, isolates TR1, TR2, TR4 and TR5. All isolates have ability to fix nitrogen and to produce IAA. Isolate TR5 has the highest ability of nitrogen fixing (1 mg/L). Isolate TR1 produce maximum IAA concentration (793,55 µg/mL) at 48 hours. Isolate TR4 has the highest ability to solubilize phosphate (31,28 ppm) with index of phosphate solubization 1,21. Isolate TR1, TR4 and TR5 are potential as biofertilizer agents.
Keywords: Biofertilizer, IAA, nitrogen fixation, phosphate, rhizosphere
Â
References
Tamil Nadu Agricultural University. 2008. Organic Farming : Biofertilizers Technology. http: // agritech.tnau.ac.in /org_farm/orgfarm_biofertilizertechnology.html. Diakses 23 Desember 2014.
Dey, P.M dan Harborne, J.B. 1997. Plant Biochemistry. Academic Press. London
Sylvia, D. M, Fuhrmann, J.J, Hartel P.G dan Zuberer, D.A. 1999. Principles and applications of soil microbiology. Prentice Hall. New Jersey.
Hidayat, A.T. 2009. Potensi Pelepasan N-NH4+ dan N-NO3- Tanah andisol yang ditanami sayuran di daerah dataran tinggi. Skripsi. Institut Pertanian Bogor. Bogor.
Latt, Z.K., Yu, S.S dan Lynn, T.M. 2013. Enhancement of Cellulolytic Nitrogen Fixing Activityof Alcaligenes sp. by MNNG Mutagenesis. International Journal of Innovation and Applied Studies. 4 (3): 979 - 986.
Singh,P. Kumar,V dan Agrawal, S. 2014. Evaluation of Phytase Producing Bacteria for Their Plant Growth Promoting Activities.http://www.hindawi.com/journals/ijmicro/2014/426483/. Diakses 26 November 2014.
Andanawarih,S. 2008. Optimasi produksi Asam Indolasetat oleh Rhizobium sp. dalam medium serum lateks hevea brasiliensis dengan suplementasi triptofan. http://repository.ipb.ac.id/bitstream/handle/123456789/18016/G08san.pdf?sequence=2. Diakses 20 Desember 2014.
Datta C. dan Basu, P.S. 2000. Indole acetic acid production by a Rhizobium species from root nodules of a leguminois shrub, Cajanus cajan. Microbiology Research. 155 :123-127.
Patten, C.L dan Glick, B.R, 2002. Regulation of indoleacetic acid production in Pseudomonas putida Gr12-2 by tryptophan and the stationary phase sigma factor Rpos. Can J microbiol 48 (7) :42-635.
Prakash, P. dan Karthikeyan, B. 2013. Isolation and Purification of Plant growth Promoting Rhizobacteria (PGPR) from the Rhizosphere of Acorus calamus grown soil. Indian Streams Research Journal. 7 (3) : 1-11.
Thakuria, D., Talukdar, N.C, Goswami, C., Hazarika, S., Boro, R.C dan Khan , M.R. 2004. Charcterization and screening of bacteria from rhizosphere of rice grown in acidic soils of Assam. Current Science. 7 (86) : 978-985.
Tripti, Kumar, V. dan Anshumali. 2012. Phosphate Solubizing Activity of Some Bacterial Strains Isolated from Chemical Pesticide Exposed Agriculture Soil. International Journal of Engginering research and Development. 3 (9) : 1-6.
Goldstein, A.H. 1995. Recent Progress in understanding the molecular genetics and biochemistry of calcium phosphate solubization by Gram negative bacteria. Biological Agriculture and Horticulture. 12 : 185-193.
Elfiati, D. 2005. Peranan Mikroba Pelarut Fosfat Terhadap Pertumbuhan Tanaman. http://library.usu.ac.id/download/fp/hutan-deni%20elfiati.pdf. Diakses 22 November 2014
Downloads
Published
Issue
Section
License
Copyright and Attribution:
Articles in Biotropika: Journal of Tropical Biology are under Creative Commons Attribution-NonCommercial (CC-BY-NC) copyright. The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to the automatic transfer of the copyright to the publisher.
Journal of Biotropika: Journal of Tropical Biology is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0).
Permissions:
Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from one of the authors.
Ethical matters:
Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the author's own must have had the correct authorization for this exportation.
