Single Cell Protein Production using Yeast Isolates from Soy Factory Waste by Co-culture Method

Chintya Corin, Tri Ardyati

Abstract


Nutrient content in soy waste able to allow growth of yeasts. Yeast isolates have potency to be used in the production of single cell protein. The research was carried out to study isolates for production of single cell protein using co-culture method and to determine the effect of co-culture method in protein content obtained. The isolates used were I2YP5K1, I2YP5K2, KYP6K1, KYP3K2, AYP6K1, AYP6K2, and AYP5K4. Steps used in this research were grown the yeast isolates in Yeast Malt Broth, assay of antagonist among yeast isolates, single cell protein production, and identification using API 20C AUX. Parameters measured were biomass, number of cells, and protein content. Design of research using two-way ANOVA with three replications. Isolates able to be used for co-culture method in the production of single cell protein were KYP3K2 (Saccharomyces cereviseae), AYP6K1, AYP6K2, and AYP5K4. Co-culture methods does not increase biomass and number of cells, however able to increase the protein content. Protein production by co-culture isolates KYP3K2 and AYP6K2 is highest than other co-culture isolates with protein content 1.31 mg / g.

 

Key words : Co-culture, single cell protein, soy waste, yeast.


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References


Abulhamd, A. T., M. M. Mokhtar, & F. R. Mohame. 2007. Biochemical and Molecular Characterization of Some Yeast Isolates. J. of Agric. Sci. 15(2):315-324.

Baeza, M. E., M. A. Sanhueza, & V. H. Cifuentes. 2008. Occurrence of Killer Yeast Strains in Industrial and Clinical Yeast Isolates. Biol. Res. 41(1): 173-182.

Bhima, B., T. A. Devi, M. S. Reddy, Y. R. Reddy, & L.V. Rao. 2011. Optimized protein extraction from yeast (Saccharomyces cerevisiae) for 2-D gel electrophoresis. J. of Theo. & Exp. Biol. 8(1):77-84.

Jiang, L. 2009. Comparison of Disk Diffusion, Agar Dilution, and Broth Microdilution for Antimicrobial Susceptibility Testing of Five Chitosans. Fujian Agricultural and Forestry University. Fujian. Tesis.

Lay, B.W. & H. Sugyo. 1992. Mikrobiologi. Rajawali Press. Jakarta.

Lee, B.K. & K. J. Kyun. 2001. Production of Candida utilis biomass on molasses in different culture types. Aquacultural Engineering, 25(1):111-124.

Lubis, H. 2006. Pengolahan Limbah Pabrik Kecap Menjadi Etanol. Pascasarjana Universitas Sumatera Utara. Medan. Tesis.

Sidauruk, H., T. Ardyati, & O. Sjofjan. 2013. The Amino Acid Profile of Yeast from Ketchup Factory Waste as a Candidate of Single Cell Protein (SCP). Int. J. Biosci. 3(9): 36-43.

Tesfaw, A. & F. Assefa. 2014. Co-culture: A Great Promising Method in Single Cell Protein Production. Biotech. & Mol. Biol. Review. 9(2): 12-20.

Waites, M. J., N. L. Morgan, J. S. Rockey, & G. Higton. 2001. Industrial Microbiology: Introduction. Blackwell Science. USA.

Widyati, E. & W., Yanti. 1996. Limbah untuk Pakan Ternak. Agrisarana. Surabaya.


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