Comparison of Lysis Buffers for Saliva DNA Extraction using Modified Magnetic-Silica Nanoparticle Beads

Authors

  • Muhammad Fauzi Arif Mulawarman University
  • Ahadi Damar Prasetya Research Organization for Nuclear Energy, National Research and Innovation Agency. Puspiptek Area, Serpong, South Tangerang 15314, Indonesia.
  • Indra Lesmana Laboratory Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Teknika Selatan Street, Sekip Utara, Yogyakarta 55281, Indonesia
  • Niken Satuti Nur Handayani Laboratory Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Teknika Selatan Street, Sekip Utara, Yogyakarta 55281, Indonesia

DOI:

https://doi.org/10.21776/ub.biotropika.2023.011.01.01

Keywords:

beads, DNA concentration, DNA extraction, lysis buffer, nanoparticles

Abstract

Our previous study revealed that modified MAGSi (magnetic-silica nanopartivel beads) had various sizes and shapes that affected the DNA extraction process. In this study, we combined our MAGSi with several kinds of lysis buffer for DNA extraction to analyze the result. Human saliva DNA was extracted using three kinds of MAGSi. Each of them was made differently. We used ATL buffer, Triton Buffer, and without buffer for cell lysis. The concentration and purity of the extracted DNA were analyzed using spectrophotometry. Extracted DNA also was used as template DNA for DNA amplification using beta globin gene. This study revealed that our MAGSi-1 provided the best result for DNA concentration and purity. This study also revealed that DNA extraction using lysis buffer produced less concentration yield and purity value but it was more consistent. The concentration and purity value were different but consistent. All the extraction can be used as DNA template for amplification of human beta globin gene. Further studies are still needed to determine the effectiveness and consistency of the lysis buffer and our MAGSi.

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Published

2023-05-23

Issue

Vol. 11 No. 1 (2023)

Section

Articles

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Copyright (c) 2023 Biotropika: Journal of Tropical Biology

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