Effect of Aeration Period and Co-Culture with Bacteria on Growth and Lipid Profile of Chlorella vulgaris
DOI:
https://doi.org/10.21776/ub.biotropika.2024.012.02.02Keywords:
aeration period, Chlorella vulgaris, co-culture, growth, lipid profileAbstract
Giving bacteria to microalgae culture can increase the growth of microalgae. Giving aeration can increase oxygen levels in the culture. Good culture conditions can optimize microalgae growth, so the biomass obtained is also higher. The amount of microalgae biomass is directly proportional to the amount of lipids produced. This study aims to determine the effect of the aeration period and the addition of potential bacteria on the growth and lipid profile of Chlorella vulgaris. The aeration periods were 6, 12, 18, and 24 hours. The bacteria used in the co-culture treatment of this study were indole acetic acid (IAA) producing bacteria, amylolytic bacteria, and nitrogen-fixing bacteria from the exploration and identification of several lakes in East Java. Microalgae C. vulgaris co-cultured with IAA, amylolytic and nitrogen-fixing bacteria were given aeration for 6 hours, 12 hours, 18 hours and 24 hours (in different treatment bottles). Microalgae treatment without giving bacteria is done only by giving aeration for 6 hours, 12 hours, 18 hours, and 24 hours (in different treatment bottles). The results showed that there was effect of the aeration period, the culture type, and the interaction between the aeration period and the addition of bacteria on the growth and lipid profile of C. vulgaris. The highest average cell density of C. vulgaris on day 16 was TBA18 mono-culture treatment with an aeration period of 18 hours (26.95 x 106 cells/mL), and BTA co-culture treatment without aeration (12.75 x 106 cells/mL). The harvested biomass of C. vulgaris was used for lipid profile assays. The results of the GC-MS test of TBA18 and BTA treatment showed that the two samples had the same six main compounds but different concentrations. The six compounds are oleic acid, palmitoleic acid, ?-linolenic acid, linoleic acid, stearidonic acid and myristic acid.
References
Rittman BE (2008) Opportunities for renewable bioenergy using microorganisms. Biotechnol. Bioeng. 100: 203-212.
Schenk PM, Thomas-Hall SR, Stephens E, Marx UC, Mussgung JH, Posten C, Kruse O, & Hankamer B (2008) Second generation biofuels: high-efficiency microalgae for biodiesel production. Bioenergy Res. 1: 20-43.
Verma NH, Shakti M, Amitesh S, Bhartendu NM (2010) Prospective of biodiesel production utilizing microalgae as the cell factories: A comprehensive discussion. African Journal of Biotechnology 9(10): 1402–1411.
Ramaraj R, Unpaprom Y, Dussadee N (2016) Cultivation of green microalga, Chlorella vulgaris for biogas purification. International Journal of New Technology and Research (IJNTR) 2(3): 117-122
Ugwu CU, Aoyagi H (2012) Microalgal culture systems: an insight into their designs, operation and applications. Biotechnology 11(3): 127-132.
Mujtaba G, Lee K (2016) Advanced treatment of wastewater using symbiotic co-culture of microalgae and bacteria. Appl. Chem. Eng 27(1): 1-9.
Prabaningtyas S, Witjoro A, Suarsini E, Aridowi D, Safitri Y, Sulistiana, Aribah D, Yanis (2019) The influence of dominant bacteria from various lakes of East Java, Indonesia on Chlorella sp. culture. IOP Conf. Ser.: Earth Environ. Sci. 276(012045).
Hartono (2014) Ekskresi amonium pada bakteri penambat nitrogen dan pengaruhnya terhadap pertumbuhan tanaman. Jurnal Bionature 15(1): 35-40.
Rodiansyah A, Mahmuda AF, Ulfah MM, Rohmawati U, Listyorini D, Suyono EA, Prabaningtyas S (2021) Identification of potential bacteria on several lakes in East Java, Indonesia based on 16S rRNA sequence analysis. Hayati J Biosci 28(2): 136-143.
Nafisah W, Prabaningtyas S, Witjoro A, Saptawati RT, Rodiansyah A (2022) Exploration non-symbiotic nitrogen-fixing bacteria from several lakes in East Java, Indonesia. Biodiversitas 23(4): 1752-1758.
Richmond A (2004) Biological principles of mass cultivation. In: Richmond A, editor: Handbook of microalgae culture: Biotechnology and applied phycology, Blackwell, pp:125-177.
Surani, Sari DK (2019) Variasi jenis pelarut dan waktu pengadukan dalam proses ekstrasi asam lemak dari mikroalga. Jurnal Inovasi dan Pengelolaan Laboratorium.
Saadudin E, Fitri SR, Wargadalam VJ (2011) Karakteristik asam lemak mikroalga untuk produksi biodiesel. Ketenagalistrikan dan Energi Terbarukan (10)2: 131 – 140.
Hadiyanto H, Azimatun NMM, Hartanto GD (2012) Cultivation of Chlorella sp. as biofuel sources in Palm Oil Mill Effluent (POME). International Journal of Renewable Energy Development 1(2): 45-49.
Sparkman O, Penton Z, Kitson FG (2011). Gas chromatography and mass spectrometry: a practical guide. Elsevier. 10.1016/C2009-0-17039-3.
David GW (2005) Analisis Farmasi. Edisi Kedua. EGC, Jakarta.
Prihartini NB, Putri B, Yuniati R (2005) Pertumbuhan Chlorella spp. dalam medium ekstrak tauge (MET) dengan variasi pH awal. Makara Sains 9(1): 1-6.
Ratnawati R, Slamet A, Hermana J (2008) Efek penambahan unsur kalium dan aerasi terhadap kinerja alga-bakteri untuk mereduksi polutan pada air Boezem Morokrembangan, Surabaya. Seminar Nasional VIII Teknik Lingkungan ITS dan Seminar Nasional VII VII Ikatan Alumni Teknik Penyehatan Indonesia (IATPI).
Kumar A, Ergas S, Yuan X, Sahu A, Zhang Q, Dewulf J, Malcata FX, van Langenhove H (2010) Enhanced CO2 fixation and biofuel production via microalgae: recent developments and future directions. Trends Biotechnol 28: 371-380.
Lirofiatillah, Prabaningtyas S, Saptasari M (2020) Effect of differences in the form of photobioreactor prototypes and aeration period on Chlorella sp. cell growth in co-culture with bacteria. AIP Conference Proceedings 2231 (040052).
Liang QU, Renjun W, Peng Z, Ruinan C, Wenli Z, Liuqing Z, Xuexi T (2014) Interaction Between Chlorella vulgaris and Bacteria: Interference and Resource Competition. Acta Oceanol. Sin. 33(1): 135-140.
Wang Y, Yang Y, Ma F, Xuan L, Xu Y, Huo H, Zhou D & Dong S (2015) Optimization of Chlorella vulgaris and bioflocculant-producing bacteria co-culture: enhancing microalgae harvesting and lipid content. Letters in Applied Microbiology 60: 497-503.
Safi C, Zebib B, Merah O, Pontalier PY, Vaca-Garcia C (2014) Morphology, composition, production, processing and applications of Chlorella vulgaris: a review. Renew Sustain Energy Rev. 35: 265-278.
Rooney-Varga JN, Giewat MW, Savin MC (2005) Links between phytoplankton and bacterial community dynamics in a coastal marine environment. Microbial Ecology 49: 163-175.
Zoppini A, Puddu A, Fazzi S (2005) Extracellular enzyme activity and dynamics of bacterial community in mucilaginous aggregates of the Northern Adriatic Sea. Science of the Total Environment 353: 270-286.
Trabelsia A, Rassoulzadegana F (2011) Inorganic nutrient control of dissolve organic carbon (DOC) dynamics in NW Mediterranea Waters: an experimental approach. Marine Biology Research 7: 667-676.
Al Asy’ari AA, Wijanarko A, Suryadi H (2013) Pengaruh penambahan bakteri Azospirillum brasilense dalam kultur campuran terhadap pertumbuhan Chlorella vulgaris. Universitas Indonesia. Undergraduate thesis.
Bai X, Lant P, Das K (2015) The contribution of bacteria to algal growth by carbon cycling. Biotechnology and Bioengineering 112(4): 688-695.
Elystia S, Saragih LR, Muria SR (2021) Algal-bacterial synergy for lipid production and nutrient removal in tofu liquid waste. International Journal of Technology 12(2): 287-297.
Rusdiani RR, Boedisantosi R, Hanif M (2016) Optimalisasi teknologi fotobioreaktor mikroalga sebagai dasar perencanaan strategi mitigasi gas CO2. Jurnal Teknik ITS 5(2): 188-192.
Hartati R, Endrawati H, Mamuaja J (2013) Fatty acid compisition of marine microalgae in Indonesia. Journal of Tropical Biology and Conservation 10: 75-82.
Knothe G (2012) Fuel properties of highly polyunsaturated fatty acid methyl esters: prediction of fuel properties of algal biodiesel. Energy Fuels 26(8): 5265-73.
Knothe G (2009) Improving biodiesel fuel properties by modifying fatty ester composition. Energy Environ Sci 2: 759-66.
Moser BR (2009) Biodiesel production, properties, and feedstock. Vitr Cell Dev Biol-Plant 45: 229-66.
Yeh KL, Chang JS (2012) Effect of cultivation conditions and media composition on cell growth and lipid productivity of indigenous microalgae Chlorella vulgaris ESP-31. Bioresour Technol 105: 120-7.
Zheng H, Yin J, Gao Z, Huang H, Ji X, Dou C (2011) Disruption of Chlorella vulgaris cells for the release of biodiesel-producing lipids: a comparison of grinding, ultrasonication, bead milling, enzymatic lysis, and microwaves. Appl Biochem Biotechnol 164: 1215-24.
Yeh KL, Chang JS (2011) Nitrogen starvation strategies and photobioreactor design for enhanching lipid content and lipid production of a newly isolated mocroalgae Chorella vulgaris ESP-31: implications for biofuels. Biotechnol. J. 6: 1358-66.
Stephenson AL, Dennis JS, Howe CJ, Scott SA, Smith AG (2009) Influence of nitrogen-limitation regime on the production by Chlorella vulgaris of lipids for biodiesel feedstock. Biofuel 1:47-58.
Chen W, Sommerfeld M, Hu Q (2011) Microwaved-assisted nile red method for in vivo quantitation regime of neutral lipids in micoalgae. Bioresour. Technol. 102: 135-41.
Jahromi KG, Koochi ZH, Kavoosi G, Shahsavar A (2022) Manipulation of fatty acid profile and nutritional quality of Chlorella vulgaris by supplementing with citrus peel fatty acid. Scientific Report 12: 8151.
Idris NA (2017) Cultivation of microalgae in medium containing palm oil mill effluent and its conversion into biofuel. Journal of Oil Palm Research 29(2): 291-299.
Knothe G (2008) Designer biodiesel: optimizing fatty ester composition to improve fuel properties. Energy and Fuels 22: 1358-1364.
Akbar E, Yaakob Z, Kamarudin SK, Ismail M, Salimon J (2009) Characteristic and composition of Jatropha curcas oil seed from Malaysia and its potential as biodiesel feedstock. European Journal Of Scientific Research 29: 396-403.
Emil A, Yaakob Z, Kumar MNS, Jahim JM, Salimon J (2010) Comparative evaluation of physicochecimal properties of Jatropha seed oil from Malaysia, Indonesia, and Thailand. Journal of the American Oil Chemists’ Society 87: 689-695.
Saravanan S, Nagarajan G (2011) Effects of single double bond in the fatty acid profile of biodiesel on its properties as a Cl engine fuel. International Journal of Energy and Environment 2: 1141-1146.
Sokoto M, Hassan L, Dangoggo S, Ahmad H Uba A (2011) Influence of fatty acid methyl esters on fuel properties of biodiesel produced from the seeds oli of Curcubita pepo. Nigerian Journal Of Basic And Applied Sciences 19: 81-86.
Kinast JA (2003) Production of biodiesels from multiple feedstocks and properties of biodiesels and biodiesels/diesels blends. National Renewable Energy Lab., Golden, CO pp. 57.
Knothe G (2005) Dependence of biodiesel fuel properties on structure of fatty acid alkil esters. Fuel Processing Technology 86: 1059-1070.
Hoekman SK, Broch A, Robbins C, Ceniceros E, Natarajan M (2012) Review of biodiesel composition, properties, and specifications. Renewable and Sustainable Energy Reviews 16: 143-169.
Rushan NH, Yasin NHM, Said FM, Ramesh N (2020) Immobilised Chlorella vulgaris as an alternative for the enhancement of microalgae oil and biodiesel production. Buletin of Chemical Reaction Engineering & Catalysis 15(2): 379-3.
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