Potential of Combination <i>Marsilea crenata</i> and <i>Curcuma xanthorriza</i> to Improve Sperm Quality of Male Mice Exposed by Monosodium Glutamate
DOI:
https://doi.org/10.21776/ub.biotropika.2022.010.01.04Keywords:
curcuma, monosodium glutamate, sperm quality, water cloverAbstract
This study aims to determine the potential of water clover (M. crenata), curcuma (C. xanthorriza), and the combination of both extracts in order to improve the sperm quality of mice after MSG administration. This study used 35 Balb/C male mice (3 months old, 25-30 grams body weight (BW)). The animals randomly divided into 7 treatment groups, namely K0 (the group given MSG 4 mg/gBW), K1 (the group given MSG 4 mg/gBW and M. crenata 0.09 mg/gBW), K3 (the group given MSG 4 mg/gBW and C. xanthorriza 0.2 mg/gBW), K4 (the group that was given MSG 4 mg/gBW and a combination of M. crenata extract 0.045 mg/gBW and C. xanthorriza 0.1 mg/gBW), K5 (the group given M. crenata 0.09 mg/gBW), and K6 (the group given C. xanthorriza 0.2 mg/gBW). MSG and all extracts are given orally and daily for 30 days. The observed parameters were sperm’s motility, viability, and concentration. The data are analyzed using SPSS for windows with a One-way ANOVA test (p≤0.05) and Tukey HSD test. The administration of water clover, curcuma, and the combination of both extracts can significantly improve the sperm quality in mice exposed by MSG. The administration of single and combination extract can improve the motility, viability, and concentration of the sperm in treatment groups with the extract. In conclusion, the combination of water clover and curcuma ethanol extract significantly improved sperm quality.
References
Anzila I, Marhendra APW, Rahayu S (2019) The effect of Monosodium L-Glutamate (MSG) treatment for short and long terms to the semen quality of adult male rats. The Journal of Experimental Life Sciences 9(2): 116–121.
Anzila I (2019) Potensi semanggi air (Marsilea crenata) dalam memperbaiki spermatogenesis dan kualitas sperma tikus (Rattus norvegicus) setelah pemberian monosodium glutamat (MSG). Tesis. Universitas Brawijaya
Rahayu S, Annisa R, Anzila I, Yuyun IC, Aris S, Agung PW & Muhammad SD. (2021) Marsilea crenata ethanol extract prevents monosodium glutamate adverse effects on the serum levels of reproductive hormones, sperm quality, and testis histology in male rats. Veterinary World 14(6): 1529–1536.
Giovambattista A, Suescun MO, Nessralla CCDL et al. (2003) Modulatory effects of leptin on leydig cell function of normal and hyperleptinemic rats. Neuroendocrinology 78(5): 270–279.
Oduwole OO, Peltoketo H, Huhtaniemi IT (2018) Role of follicle-stimulating hormone in spermatogenesis. Frontiers in Endocrinology 9 (December): 1–11.
Jayaprakasha GK, Jaganmohan Rao L, Sakariah KK (2006) Antioxidant activities of curcumin, demethoxycurcumin and bisdemethoxycurcumin. Food Chemistry 98 (4): 720–724.
Sakr SA, Badawy GM (2013) Protective effect of curcumin on monosodium glutamate-induced reproductive toxicity in male albino rats. Global Journal of Pharmacology 7(4): 416–422.
Ngadino, Setiawan, Koerniasari, Ernawati & Sudjarwo SA. (2018) Evaluation of antimycobacterial activity of Curcuma xanthorrhiza ethanolic extract against Mycobacterium tuberculosis H37Rv in vitro. Veterinary World 11(3): 368–372.
Gómez Montoto L, Magaña C, Tourmente M , Juan MC, Cristina C, Juan JL, Montserrat G & Eduardo RS. (2011) Sperm competition, sperm numbers and sperm quality in muroid rodents. PLoS ONE 6(3): 1-10.
Mulyani SSTE, Adriani M, Wirjatmadi B (2016) Effect of mung bean sprouts extract to the morphology and motility spermatozoa in mice exposed monosodium glutamate. International Journal of Preventive and Public Health Sciences 2 (3): 14–17.
Aliff MH, Nooraain H, Nurdiana S (2017) Ameliorating effects of coconut water on sperm quality and selected organs histology in monosodium glutamate pre treated male mice (Mus musculus). Malaysian Applied Biology 46(1): 27–35.
Agustina W, Widjiati W, Hayati A (2018) Effects of red fruit (Pandanus conoideus Lam) oil on malondialdehyde level and spermatozoa quality in mice (Mus musculus) exposed to monosodium glutamate. Folia Medica Indonesiana 54(2): 84.
Brookes PS, Yoon Y, Robotham JL, Anders W & Shey SS. (2004) Calcium, ATP, and ROS: A mitochondrial love-hate triangle. American Journal of Physiology - Cell Physiology 287: C817-C833.
Turner TT, Lysiak JJ (2008) Oxidative stress: A common factor in testicular dysfunction. Journal of Andrology 29(5): 488–498.
Bouayed J, Bohn T (2010) Exogenous antioxidants - Double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxidative Medicine and Cellular Longevity 3(4): 228–237.
Tomar R, Jain AK, Mohanty NK, Choudhary R, Chawala VK & Soni ND. (2008) Role of oxidative stress and antioxidants in male infertility. Toxicology Letters 180(2): S204.
Rosidi A, Khomsan A, Setiawan B, Hadi R & Dodik B. (2016) Antioxidant potential of temulawak (Curcuma xanthorrhiza Roxb). Pakistan Journal of Nutrition 15(6): 556–560.
Jagetia GC, Rajanikant GK (2015) Curcumin stimulates the antioxidant mechanisms in mouse skin exposed to fractionated γ-irradiation. Antioxidants 4(1): 25–41.
Tourmente M, Villar-Moya P, Rial E, Roldan ERS (2015) Differences in ATP generation via glycolysis and oxidative phosphorylation and relationships with sperm motility in mouse species. Journal of Biological Chemistry 290(33): 20613–20626.
Gill SS, Pulido OM (2001) Review article: glutamate receptors in peripheral tissues: current knowledge, future research, and implications for toxicology. Toxicologic Pathology 29(2): 208–223.
Agarwal A, Virk G, Ong C, du Plessis SS (2014) Effect of oxidative stress on male reproduction. The World Journal of Men’s Health 32(1): 1.
Ismail El-Sawy HB, Soliman MM, El-Shazly SA, Ali HAM (2018) Protective effects of camel milk and Vitamin E against monosodium glutamate induced biochemical and testicular dysfunctions. Progress in Nutrition 20(1): 76–85.
Omu AE, Al-Azemi MK, Kehinde EO, Anim JT & Oriowo MA. (2008) Indications of the mechanisms involved in improved sperm parameters by zinc therapy. Medical Principles and Practice 17(2): 108–116.
Soleimanzadeh A, Saberivand A (2013) Effect of curcumin on rat sperm morphology after the freeze-thawing process. Veterinary Research Forum 4(3): 185–189.
Lu WP, Mei XT, Wang Y, Yan PZ, Yun FX & Dong HX. (2015) Zn(II)-curcumin protects against oxidative stress, deleterious changes in sperm parameters and histological alterations in a male mouse model of cyclophosphamide-induced reproductive damage. Environmental Toxicology and Pharmacology 39(2): 515–524.
Lesmana R (2019) Combination Formulated Herbals (Pimpinella alpina, Eurycoma longifolia, and Curcuma xanthorrhiza) increases number and improves the quality of sperm in male rat. International Journal of Pharma Medicine and Biological Sciences 8(3): 106–109.
Kianifard D (2015) Protective effect of Morus alba extract on the alteration of testicular tissue and spermatogenesis in adult rats treated with monosodium glutamate. Medicine Science 4(1): 1947–1958.
Patricio A, Cruz DF, Silva JV, Ana P, Barbara RC, Luis KG, Rita F, Nuno M, Saul A, Joao L, Vladimiro S & Margarinda F. (2016) Relation between seminal quality and oxidative balance in sperm cells. Acta Urológica Portuguesa 33(1): 6–15.
Hogarth CA, Griswold MD (2010) The key role of vitamin A in spermatogenesis. Journal of Clinical Investigation 120(4): 956–962.
Belhan S, Yıldırım S, Huyut Z, Ugur O, Gokhan O & Sermin A. (2020) Effects of curcumin on sperm quality, lipid profile, antioxidant activity and histopathological changes in streptozotocin-induced diabetes in rats. Andrologia 52(6): 1–8.
Downloads
Published
Issue
Section
License
Copyright and Attribution:
Articles in Biotropika: Journal of Tropical Biology are under Creative Commons Attribution-NonCommercial (CC-BY-NC) copyright. The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to the automatic transfer of the copyright to the publisher.
Journal of Biotropika: Journal of Tropical Biology is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0).
Permissions:
Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from one of the authors.
Ethical matters:
Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the author's own must have had the correct authorization for this exportation.
