Pengaruh Gula Reduksi dan Total Nitrogen Terhadap Densitas dan Viabilitas Sel Saccharomyces cerevisiae dalam Fermentasi Etanol dari Molase

Authors

  • ekwan nofa wiratno
  • Tri Ardyati

Abstract

Fermentasi etanol dipengaruhi oleh beberapa faktor, salah satu diantaranya adalah densitas dan viabilitas sel khamir. Penelitian ini bertujuan mengetahui pengaruh variasi total nitrogen dan gula reduksi terhadap densitas dan viabilitas sel Saccharomyces cerevisiae (SAF Instan) selama proses fermentasi etanol dari molase. Tahapan penelitian ini terdiri dari pengukuran kadar gula reduksi, total nitrogen, konsentrasi kalsium, penentuan densitas dan viabilitas sel khamir serta analisis data. Fermentasi dilakukan selama 72 jam dengan 3 ulangan. Perlakuan konsentrasi gula reduksi (GR) dan total nitrogen (N) yaitu GR 100 N 0, GR 100 N 6, GR 100 N 10, GR 125 N 0, GR 125 N 6 dan GR 125 N 10 g/l. Penghitungan viabilitas sel dilakukan setiap 24 jam. Viabilitas tertinggi adalah GR 125 N 0 (95,76 %) dan GR 100 N 6 (95,96 %). Penurunan viabilitas sel disebabkan oleh rendahnya perkembangbiakan sel khamir (tidak terjadi peningkatan jumlah sel secara signifikan (p>0,05)). Hal ini terjadi karena tingginya konsentrasi kalsium (0,21 %).

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Published

2013-05-01

Issue

Vol. 1 No. 1 (2013)

Section

Articles

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