Pengaruh Ekstrak Ethanol Kemangi (Ocimum canum Sims.) terhadap Struktur Histologi Testis Mencit (Mus musculus) Jantan
DOI:
https://doi.org/10.21776/ub.biotropika.2017.005.01.4Abstract
Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak ethanol kemangi (Ocimum canum Sims.) terhadap struktur histologi testis mencit jantan (Mus musculus). Penelitian ini menggunakan 9 ekor hewan coba mencit strain Balb/c jantan berumur 3 bulan, berat badan 20 - 30 g. Hewan coba dibagi menjadi tiga (3) kelompok kontrol tanpa pemberian ekstrak kemangi (P0), kelompok yang diberi ekstrak kemangi dengan dosis 100 mg/kgBB (P1) dan 200 mg/kgBB (P2). Ekstrak kemangi diberikan setiap hari secara oral selama 35 hari. Parameter yang diamati adalah diameter tubulus seminiferus, tebal epitel germinal, jumlah lapisan germinal dan jumlah sel spermatogenik meliputi spermatogonia, spermatosit dan spermatid. Data yang diperoleh dianalisis dengan One-way ANOVA (p ≤ 0,05). Jika menunjukkan perbedaan yang bermakna, maka dilanjutkan dengan uji Least Significant Difference (LSD). Hasil penelitian menunjukkan bahwa pemberian ekstrak kemangi dosis 200 mg/kgBB meningkatkan diameter tubulus seminiferus, tebal epitel germinal, dan jumlah sel spermatidReferences
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