Identifikasi Padi Lokal Jawa Timur Berdasarkan Gen BADH2 yang Berpotensi sebagai Padi Beraroma
DOI:
https://doi.org/10.21776/ub.biotropika.2018.006.03.04Keywords:
Padi Lokal, Jawa Timur, BADH2Abstract
Padi lokal merupakan padi liar yang telah dibudidayakan oleh petani Indonesia. Potensi padi lokal dapat digunakan sebagai plasma nutfah yang memiliki berbagai gen dalam mengendalikan sifat tertentu. Beberapa beras di Indonesia memiliki potensi sebagai beras aromatik. Berbagai varietas padi aromatik berpotensi meningkatkan kualitas beras di Indonesia. Aroma beras dikendalikan oleh gen betain aldehid dehidrogenase (BADH). Gen ini terdiri dari dua alel yaitu BADH1 dan BADH2, mengkodekan enzim yang bertanggung jawab untuk mengkatalisis sintesis senyawa 2-acetyl-1-pyrroline (2-AP). Penelitian ini bertujuan untuk mengidentifikasi potensi aroma varietas Berlian. Gen BADH2 diisolasi dari daun pada ketiga varietas tersebut dan diamplifikasi menggunakan primer forward 5'-TTGTTTGGCTCTTGCTGATG -3' dan primer reverse 5'CATAGGAGCAGCTGAAATATATACC-3'. Fragmen gen BADH2 berhasil diamplifikasi dengan ukuran 258 bp (varietas Berlian). Sekuen BADH2 varietas Berlian menunjukkan kemiripan 100% dengan sekuen BADH2 padi tidak beraroma dan tidak memiliki kemiripan dengan sekuen BADH2 varietas padi beraroma. Analisis potensi padi beraroma menunjukkan bahwa varietas Berlian tidak berpotensi sebagai padi beraroma.
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