Varietas Padi Lokal Jawa Timur Tahan Cekaman Kekeringan Berdasarkan Gen DREB2A
DOI:
https://doi.org/10.21776/ub.biotropika.2018.006.03.03Keywords:
Cekaman Kekeringan, Varietas Padi Lokal, Gen DREB2A, Domain ERFAbstract
Padi (Oryza sativa L.) adalah tanaman pangan utama masyarakat Indonesia. Masalah kekeringan dalam upaya penanaman padi adalah hal yang biasa di kalangan petani. Penelitian tahan cekaman kekeringan pada padi perlu dilakukan untuk mengatasi masalah ketersediaan pangan. Sifat tahan cekaman kekeringan pada tanaman salah satunya dikendalikan oleh gen DREB2A. Varietas padi lokal Indonesia untuk tahan cekaman kekeringan belum banyak diteliti. Penelitian ini bertujuan untuk mempelajari varietas padi lokal Jawa Timur yang tahan terhadap cekaman kekeringan menggunakan gen DREB2A, yang diisolasi menggunakan primer forward 5’-CCT CAT TGG GTC AGG AAG AA-3’ dan primer reverse 5’-GGA TCT CAG CCA CCC ACT TA-3’. Penelitian ini menggunakan tiga varietas padi lokal, yaitu Berlian dan SOJ A3, keduanya dari Banyuwangi, dan varietas Jawa dari Malang. Isolasi DNA total dilakukan mengikuti protokol Kit Macherey-Nagel. Amplifikasi gen DREB2A dilakukan menggunakan metode Polymerase Chain Reaction (PCR). Fragmen gen DREB2A berhasil diamplifikasi sepanjang 239 bp (varietas Berlian), 239 bp (varietas SOJ A3), dan 240 bp (varietas Jawa). Analisis sampel gen DREB2A varietas padi lokal Jawa Timur menunjukkan hilangnya kemampuan gen DREB2A dari varietas Jawa untuk mengikat cis-regulator elemen promotor gen target.
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