Effectivity Combination of Bitter Melon (Momordica charantia) and Bitter (Andrographis paniculata) Extract to Suppress Proinflammatory Cytokines in Diabetic Mouse Models

Authors

  • Firda Agustin Brawijaya University
  • Muhaimin Rifa'i

Abstract

nsulin Resistance (IR) is main characteristic of Type 2 Diabetes Mellitus. IL-6 and IFNγ play important role in the deterioration of this disease. The aim of this experiment was to know effectivity combination of Bitter Melon and Bitter to decrease the expression of IL-6 and IFNγ in Type 2 Diabetes Mellitus. Type 2 Diabetes Mellitus were induced by injecting streptozotocin to neonate BALB/c mice in the age of five days (100 mg/kg BW). This experiment applied five groups which divided into  normal group, T2D (Type 2 Diabetes Mellitus model group), T2D-D1 (Bitter Melon dose 5,6 mg/kg BW and Bitter 20 g/kg BW), T2D-D2 (Bitter Melon doses 56 mg/kg BW and Bitter doses 200 mg/kg BW) and T2D-D3 (Bitter Melon doses 5600 mg/kg BW and Bitter doses 20000 mg/kg BW). Relative number of proinflammatory cytokines were analyzed using One-Way ANOVA with SPSS 16.0 for Windows. The results showed that the treatment with Bitter melon (Momordica charantia) and bitter (Andrographis paniculata) gave different effect compared to T2D groups. Medicinal herbs groups supressed proinflamatory cytokines such as IL-6 and IFNγ. So that relative number of IL-6 and IFNγ in treated group is lower than Type 2 Diabetes Mellitus (T2D) mice model.

 

Key words: IFNγ, IL-6, Insulin, Proinflammatory

Author Biography

Firda Agustin, Brawijaya University

Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University

References

. Baynes, J.W. 2003. Role of Oxidative Stress in Diabetic Complications. A new perspective on an old paradigm. J Diabetes 48:1-9.

. Rifa’i M, Widodo N. 2014. Significance of propolis administration for homeostasis of CD4+CD25+ immunoregulatory T cells controlling hyperglycemia. Springer 3 (1):1-8.

. Hotamisligil, G.S, Shargill N.S. & Spiegelman B.M. 1993. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science 259:87–91.

. Kopp H.P, Kopp C.W, Festa A, Krzyzanowska K, Kriwanek S, Minar E, Roka R. & Schernthaner G. 2003. Impact of Weight Loss on Inflammatory Proteins And Their Association with The Insulin Resistance Syndrome in Morbidly Obese Patients. Arterioscler Thromb Vasc Biol 23:1042–1047.

. Yuan M, Konstantopoulos N, Lee J, Hansen L, Li ZW, Karin M, & Shoelson S.E. 2001. Reversal of obesity- and diet-induced insulin resistance with salicylates or targeted disruption of Ikkbeta. Science. 293:1673–7.

. Rotter V, Nagaev I, & Smith U. 2003. Interleukin-6 (IL-6) induces insulin resistance in 3T3–L1 adipocytes and is, like IL-8 and tumor necrosis factor-alpha, overexpressed in human fat cells from insulin-resistant subjects. J Biol Chem 278:45777– 45784.

. Lagathu C, Bastard J.P, Auclair M, Maachi M, Capeau J, & Caron M. 2003. Chronic interleukin-6 (IL-6) treatment increased IL-6 secretion and induced insulin resistance in adipocyte: prevention by rosiglitazone. Biochem Biophys Res Commun 311:372–379.

. Shi, H. Tzameli, I. Bjorbaek, C. & Flier, J.S. 2004. Suppressor of cytokine signaling 3 is a physiological regulator of adipocyte insulin signaling. J Biol Chem 279:34733–34740.

. Samuel, C.E. 2001. Antiviral actions of interferons. Clin Microbiol Rev 14: 778–809.

. Kahn, B.B & Flier, J.S. 2000. Obesity And Insulin Resistance. J Clin Invest 106: 473–481.

. Tsutomu Wada, Masashi Hoshino , Yukari Kimura , Minoru Ojima , Tetsuro Nakano , Daisuke Koya, Hiroshi Tsuneki & Toshiyasu Sasaoka. 2011. Both type I and II IFN induce insulin resistance by inducing different isoforms of SOCS expression in 3T3-L1 adipocytes. American Journal of Physiology - Endocrinology and Metabolism. 300 (6).

. Chinedum Ogbonnaya Eleazu, Kate Chinedum Eleazu, Sonia Chukwuma & Udeme Nelson Essien. 2013. Review of the mechanism of cell death resulting from streptozotocin challenge in experimental animals, its practical use and potential risk to humans. Journal of Diabetes And Metabolic Disorders 12:60.

. Singh, N. Gupta, M. Sirohi, P. & Varsha. 2008. Effects of alcoholic extract of Momordica charantia (Linn.) whole fruit powder on the pancreatic islets of alloxan diabetic albino rats. J Environ Biol 29 (1): 101-106.

. Yu, E.C., Hung, C.R., Chen, W.C & Cheng, J.T. 2003. Antihyperglycemic Effect of Andrographolide in Streptozotocin-induced Diabetic Rats. J.Plant. Med. 69 (12): 1075-1079 .

. Guilherme .A, Virbasius J.V, Puri.V, Czech M.P. 2008. Adipocyte Dysfunctions Linking Obesity to Insulin Resistance And Type 2 Diabetes. Nat Rev Mol Cell Biol. 9 (5): 367-77.

. Hyokjoon Kwon & Jeffrey .E. Pessin. 2013. (online). Adipokines mediate inflammation and insulin resistance. http://journal.frontiersin.org. Diakses Pada 1 Oktober 2014.

. Rui, L. Yuan, M. Frantz, D. Shoelson, S. & White, M. F. 2002. SOCS-1 and SOCS-3 block insulin signaling by ubiquitin-mediated degradation of IRS1 and IRS2. J. Biol. Chem. 277: 42394–42398.

. Chao, W.W. & Lin, B.F. 2010. Isolation and Identification of Bioactive compounds in Andrographis paniculata (Chuanxinlian). Chin. Med. J. 5:1-15.

. Hafizur. R.M, Kabir.N & Chishti S. 2011. Modulation of pancreatic β-cells in neonatally streptozotocin-induced type 2 diabetic rats by the ethanolic extract of Momordica charantia fruit pulp. Natural Product Research 25 (4).

. Marc Y. Donath. 2014. Targeting Inflammation in the Treatment of Type 2 Diabetes: Time to Start. Nature Reviews Drug Discovery 13: 465–476.

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Published

2015-06-12

Issue

Vol. 3 No. 2 (2015)

Section

Articles

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