THE EFFECT AND THE UTILIZATION OF X-RAY EXPOSURE AGAINTS MICRONUCLEUS FREQUENCY OF LYMPOCYTES CELLS FOR BIOLOGICAL DOSIMETER

Triesha Retno Astari, Agung Pramana Warih

Abstract


ABSTRACT

Use of nuclear technology in various fields requires a development of a simple and quick method to determine the risks of person after exposed to radiation. Micronucleus (MN) is one of the indication of structural defect on chromosome as a result of radiation exposure which can be observed in a cell with two nuclei (binucleic cells, BNC), by blocking the cleavage process using cytochalacine-B. The purpose of this study was to determine the frequency of MN that formed after radiation exposure based on gender and age of respondences (donor). Blood samples from six healthy person with age of about 25-51 years old were irradiated with X-ray at doses of 0 and 2 Gy, then MN culturing and its harvesting were done with standard procedures. The results showed that MN frequency was different with differences in age and gender of the respondents ( donors ) that were irradiated at doses of 0 Gy (control) and 2 Gy (α = 5 %). It was found that the MN frequency induced by dose of 2 Gy was significantly higher than that of 0 Gy . However, MN in each age group of 28-30 years, 42-43 years and 50-51 years were not significantly different. It is may be caused by the difference of laboratory condition and other factors such as the amount and sample condition that used with variety of ages >60 years old. The MN test methods of peripheral blood lymphocytes due to radiation enable to be used to learn the irradiation effect in in vitro on the chromosome and can be used as a biological dosimeter.

 

Key words : Biodosimetri, cytokinesis Block ( CB ), dose, micronuclei, ionizing radiation .


 


 


 


Full Text:

PDF

References


Riyanto, Agus. 2014. Risiko Pajanan Radiasi Sinar-X terhadap Pekerja Radiasi di bagian Radiologi Rumah Sakit Umum Pusat Nasional Cipto Mangunkusumo Jakarta Tahun 2014. Proposal Tesis. FKM. UI

Yanti dkk. 2007. Penerapan Efek Interaksi Radiasi Dengan Sistem Biologi Sebagai Dosimeter Biologi. PTKMR-BATAN. Jakarta

Rao, B.S. and Natarajan. A.T. 2001.Retrospective Biological Dosimetry Of Absorbed Radiation. Radiation Protection Dosimetry. 95, 17-23.

International atomic energy agency, Biological Dosimetry Chromosomal Aberration Analysis for Dose Assessments. 2001. Technical Reports Series No. 260, IAEA, Viena, 25-31

Countryman,p.i., Heddle, j.a.1979.The Production of Micronuclei from Chromosome Aberration in Irradiated Culture of Human Lymfocytes, mutation research, 41, 321-331

Fenech, M And Marley, A.A. 1985. Measurement Of Micronuclei in Limphocytes. Mutation Research Vol 147 hal 29-36

Hogsted. 1984. Micronuclei in Limphocytes with Preserved Cytoplasm, A Method For Assesment Of Cytogenetic Damage in Man. Mutation Research Vol 130 hal 62-72

Ronald,E., Walpole. 1995. Pengantar Statistika. Gramedia. Jakarta

Mill, Aj. , Well, J, Hall,C.S. And Butler, A.1996. Mikronucleus Induction in Human Lymphocytes Comparative Effects Of X Rays, Alpha Particles, Betha Perticles And Neutrons and Implications For Biological Dosimetry. Mutation Research No. 145 p 575-576

Fenech M. 2007. Protocol Cytokinesis Block Mikronukleus Cytome Assay. Nature Protocols Vol.2 No. 5 p 1084-1104

Fenech M. and Morley A.A. 2001. Cytokinesis block micronucleus method in human lymphocytes: Effect of in vivo ageing and low dose x-irradiation. Mutat. Res. 161, 193-198. 5.

Sieber OM, Heinimann K, Tomlinson IP. 2003. Genomic Instability the engine of tumorigenesis. Nat Rev Cancer

Solomon F. D. Paul, P. Venkatachalam and R.K. Jeevanram. 1997. Analysis of Radiation Dose-Response Curve Obtained with Cytokinesis Block Micronucleus Assay. Nuclear Medicine and Biology. Elsevier Science Inc.

Funda S. Pala1 , Fadime ALKAYA2 , Kıymet TABAKÇIOĞLU1 , Füsun TOKATLI2 , Cem UZAL3 . 2008. The Effects of Micronuclei with Whole Chromosome on Biological Dose Estimation. Turk J Biol. Turkey

J. Pajic, B. Rakic, D. Jovicic, A. Milovanocic. 2014. Construction of dose response calibration curves for disentrics and micronuclei for X radiation in a Serbian population. Mutation Research


Refbacks

  • There are currently no refbacks.