Activity Test of VipAlbumin® against Blood Glucose Levels and Lymfhocytes Profiles in Balbc mice as Type 2 Diabetes Mellitus model

Authors

  • Ganys Tri Silvana
  • Muhaimin Rifa'i

Abstract

Activity Test of VipAlbumin® against Blood Glucose Levels and Lymfhocytes Profiles in Balb/c mice as Type 2 Diabetes Mellitus model

 

Ganys Tri Silvana1), Muhaimin Rifa’i2)

1) Biology Department, Faculty of Science, Brawijaya University, Malang, Indonesia

 

Abstract

Diabetes mellitus (DM) has a prevalence that is increase from year to year, so we need an effective and economical drugs to cope. VipAlbumin® have high antioxidant content, so it can be used as an effective diabetes therapy. The purpose of this study was to determine the effect VipAlbumin® derived from Fish Cork (Channa striata) in decreasing blood glucose levels and assessing relative number of T cells. Mice models of  diabetes mellitus (DM) were created by injecting treptozotocin in  mice at the age of 5 days (streptozotocin in 1 ml of citrate buffer solution, a dose of 100 mg / kg BW). Mice were divided into 5 groups (Normal mice, DM, DM-D1, DM-D2 and DM-D3). After 3 weeks, the levels of blood glucose was examined and then oral therapy with VipAlbumin® was performed for 15 days. Profiles of CD4+, CD8+, and CD4+CD62L+ T cells were analyzed by flow cytometry by isolating splenic cells. One-way ANOVA was used to analyze the data. The differences between groups were considered significant at P<0.05. All results were presented as the mean of ± SD values of 5 mice in each group. In this experiment we showed that VipAlbumin® have a capability to prevent T cells activation by increasing naïve type status of both CD4 and CD8 population in mice model of DM. The number of CD4+ and CD8+ T cells decreased significantly in a dose of D3. Effective dose to decrease blood sugar in mice with type 2 diabetes is on the D3.

 

Keyword : Diabetes Melitus, Flowcytometry, Lympocytes, Streptozotocin, VipAlbumin®.

Author Biography

Ganys Tri Silvana

Biology department

References

. Syamsudin, Rita, D. Marletta& Kusmardi. 2008. Immunomodulatory and in vivo antiplasmodial activities of propolis extract. Global Journal of Pharmacology.Vol. 2, no.3. pp. 37-40.

. Katzung, Bertam G. 2002. Farmakologi Dasar dan Klinik Buku 2 Ed.8. Salemba Medika Glance. Jakarta.

. Buse JB, Polonsky KS and Burant CF. 2003, Type 2 Diabetes Mellitus, in: Williams Text Book of Endocrinology 10th Edition, 1427-1429, Saunders,USA.

. Woodley M and Whelant A. 1995. Pedoman Pengobatan. Jogjakarta: Andioffset Essensia Medica, pp: 36-39.

. Masharani, U, Karam, J.H., and German, M.S. 2004. Pancreatic Hormones & Diabetes Mellitus. In: Greenspan FS, Gardner DG. Editors. Basic & Clinical Endocrinology 7th Ed. New York: McGraw-Hill. P. 658-746.

. Baynes, J.W. 1991. Role of Oxidative Stress in Development of Complications in Diabetes. Diabetes. 40: 405-412.

. Waspadji, S. 2000. Telaah Mengenai Hubungan Faktor Metabolik dan Respon Imun pada Pasien Diabetes Melitus tipe1 : Kaitannya dengan Ulkus/gangren Diabetes. Program Pascasarjana Universitas Indonesia. JKPKBPPK/Badan Litbang Kesehatan.

. Sujudi. 2006. Buku Ajar Edisi Revisi Mikrobiologi kedokteran. Bina Rupa Aksara. Tangerang.

. Weir, D. M. 1990.Segi praktis Imunologi. Yulius ES.Jakarta: Binarupa Aksara.

. Linda K., Widyarti, S., dan Rifa’i, M. 2012. Pengaruh Pemebrian Ekstrak Etanol Daun Sirsak (Annona muricata Linn) terhadap Peningkatan Jumlah Sel T CD4+ dan CD8+ pada Timus Mencit (Mus musculus). Jurusan Biologi FMIPA UB. Malang.

. Tedder, T.F., D.A. Steeber, A. Chen, & P. Engel. 1995. The Selectins: Vascular Adhesion Molecules. FASEB J. 9, 866–873.

. Setyawan, Rudi. 2010. Pengaruh Pemberian Ekstrak Kelopak Bunga Rosela (Hibiscus sabdariffa L) Terhadap Penurunan Kadar Gula Darah Tikus Putih (Rattus norvegicus) Yang Di induksi Aloksan. Fakultas Kedokteran Universitas Sebelas Maret. Surakarta.

Downloads

Published

2015-02-24

Issue

Vol. 3 No. 1 (2015)

Section

Articles

License

Copyright and Attribution:

Articles in Biotropika: Journal of Tropical Biology are under Creative Commons Attribution-NonCommercial (CC-BY-NC) copyright. The work has not been published before (except in the form of an abstract or part of a published lecture or thesis) and it is not under consideration for publication elsewhere. When the manuscript is accepted for publication in this journal, the authors agree to the automatic transfer of the copyright to the publisher.

Journal of Biotropika: Journal of Tropical Biology is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0).

Permissions:

Authors wishing to include figures, tables, or text passages that have already been published elsewhere and by other authors are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from one of the authors.

Ethical matters:

Experiments with animals or involving human patients must have had prior approval from the appropriate ethics committee. A statement to this effect should be provided within the text at the appropriate place. Experiments involving plants or microorganisms taken from countries other than the author's own must have had the correct authorization for this exportation.