Activity Test of VipAlbumin® against Blood Glucose Levels and Lymfhocytes Profiles in Balbc mice as Type 2 Diabetes Mellitus model

Ganys Tri Silvana, Muhaimin Rifa'i

Abstract


Activity Test of VipAlbumin® against Blood Glucose Levels and Lymfhocytes Profiles in Balb/c mice as Type 2 Diabetes Mellitus model

 

Ganys Tri Silvana1), Muhaimin Rifa’i2)

1) Biology Department, Faculty of Science, Brawijaya University, Malang, Indonesia

 

Abstract

Diabetes mellitus (DM) has a prevalence that is increase from year to year, so we need an effective and economical drugs to cope. VipAlbumin® have high antioxidant content, so it can be used as an effective diabetes therapy. The purpose of this study was to determine the effect VipAlbumin® derived from Fish Cork (Channa striata) in decreasing blood glucose levels and assessing relative number of T cells. Mice models of  diabetes mellitus (DM) were created by injecting treptozotocin in  mice at the age of 5 days (streptozotocin in 1 ml of citrate buffer solution, a dose of 100 mg / kg BW). Mice were divided into 5 groups (Normal mice, DM, DM-D1, DM-D2 and DM-D3). After 3 weeks, the levels of blood glucose was examined and then oral therapy with VipAlbumin® was performed for 15 days. Profiles of CD4+, CD8+, and CD4+CD62L+ T cellswere analyzed by flow cytometry by isolating splenic cells. One-way ANOVA was used to analyze the data. The differences between groups were considered significant at P<0.05. All results were presented as the mean of ± SD values of 5 mice in each group. In this experiment we showed that VipAlbumin® have a capability to prevent T cells activation by increasing naïve type status of both CD4 and CD8 population in mice model of DM. The number of CD4+ and CD8+ T cells decreased significantly in a dose of D3. Effective dose to decrease blood sugar in mice with type 2 diabetes is on the D3.

 

Keyword : Diabetes Melitus, Flowcytometry, Lympocytes, Streptozotocin, VipAlbumin®.


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