Amplifikasi Gen COI dan 16s rRNA dari Invertebrata Laut Plakobranchus ocellatus
Abstract
ABSTRAK
Studi mengenai hubungan kekerabatan, evolusi dan identifikasi spesies secara molekuler tidak terlepas dari isolasi DNA dan amplifikasi gen COI dan 16s rRNA. Keberhasilan isolasi DNA dan amplifikasi gen COI dan 16S rRNA merupakan langkah awal yang penting untuk studi mengenai hubungan kekerabatan, evolusi dan identifikasi spesies. Penilitian ini bertujuan untuk mengamplifikasi gen COI dan 16S rRNA dari invertebrata laut yang diambil dari Raja Ampat, Plakobranchus ocellatus. Sepasang primer COI universal LCO-1490 dan HCO-2198 digunakan untuk mengamplifikasi fragmen gen COI dari P.ocellatus, sedangkan amplifikasi gen 16s rRNA menggunakan sepasang primer 16Sar-L dan 16Sbr-L. Fragmen gen COI dan 16S rRNA berhasil teramplifikasi dengan panjang amplikon masing-masing sekitar 680 bp dan 450 bp.
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Kata kunci: COI, gen, identifikasi, Plakobranchus ocellatus dan 16S rRNA
ABSTRACT
The first important step to study about organism relationship, evolution and species identification are DNA isolation and amplification of 16S rRNA and COI genes. This research aimed to amplify COI and 16S rRNA genes of marine invertebrates taken from Raja Ampat, Plakobranchus ocellatus. A pair of COI universal primer LCO-1490 and HCO-2198 was used for ampification a fragment COI gene P.ocellatus, while a fragment of the 16s rRNA gene was amplified using 16Sbr-L and 16Sar-L primer. Either  a fragment COI or 16S rRNA genes was successfully amplified, with a length amplicon about 680 bp and 450 bp, respectively.
Keywords : COI, gene, identification, Plakobrancus ocellatus, and 16S rRNA
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