Effect of Elephantopus scaber.L and Polyscias obtusa Leaf Extract for CD8+ and CD8+CD62L+ T Cell Modulation in Balb/c Mice
Abstract
Medicinal herbal is highly recommended especially for pregnant women with bacterial infection to reduce the consumption of synthetic antibiotics that are harmful to their body and also fetus. E. scaber and P. obtusa are believed to increase the number of imunocompetent cells such as CD8+ and CD8+CD62L+ T cell cause both plants have biochemical compound such as saponin and flavonoid. These plants good for immune system, but optimum dose of mixture extracts from both leaves to increase the number of CD8+ and CD8+CD62L+ T cell in mice is doesnt know yet. This study to determine effect of E. scaber and P. obtusa leaf extract by compared the number of CD8+ and CD8+CD62L+ T cell between control mice and treated mice. E. scaber and P. obtusa leaf extract can not reduce relative number of CD8+ T cells compared with control mice after 14 days treatment and 18 days. Even it show little different number but it was not significantly different, whereas the number of CD8+ decreased in K2 control mice also not significantly different than other treatments. K1 group is always show the highest number of CD8+ T cell compared to other treatments. CD8+ was decreased by increasing the doses of P. obtusa leaf extract.
Key word: E. scaber, CD8+ T cell, CD8+CD62L+ T cell, P. obtusa, Salmonella typhimurium.References
Baratawidjaja, K. G., Iris R. 2010. Imunologi Dasar Edisi Ke-10. Fakultas Kedokteran Universitas Indonesia. Jakarta
Dewi, P. 2007. Karya Tulis Ilmiah: Pengaruh Pemberian Ekstrak Lidah Buaya (Aloe vera) Terhadap Proliferasi Limfosit Pada Mencit Balb/C Yang Diinfeksi Salmonella typhimurium. FK Universitas Diponegoro. Semarang.
Fuente, M., Victor V. M. 2000. Anti-oxidants as modulators of immune function. Immunology and Cell Biology 78: 49-54.
Jan, T. R., Wey S. P., Kuan C. C., Liao M. H., Wu H. Y. 2007. Diosgenin, A Plant Derived Sapogenin, Enchange Regulatory T-cell Immunity In The Intestine Of Mice With With Food Allergy. J. PlantaMed. 73:421-426.
Juniarianto, E. 1987. Penetapan Kandungan Zat Besi Pada Akar Dan Daun Tapak Liman (Elephantopus scaber, Linn.) FF UNAIR. Surabaya.
Lee, J. K. dan Evelyn R. H. 1996. Farmacology: A Nurcing Process Approach. Penerjemah: Peter A. Gramedia. Jakarta
Linda, K., Sri W., dan Muhaimin R. 2012. Pengaruh Pemberian Ekstrak Etanol Daun Sirsak (Annona muricata Linn.) Terhadap Peningkatan Jumlah Sel T CD4+ dan CD8+ pada Timus Mencit (Mus musculus). Jurusan Biologi FMIPA Universitas Brawijaya. Malang.
Marmi. 2010. Thesis: Aktivitas Biologi Ekstrak Tapak Liman (Elephantopus scaber, L) Terhadap Perkembangan Limfosit Pada Mencit Balb/C. Jurusan Biologi FMIPA Universitas Brawijaya. Malang.
Pinca, S., Muhammad S. D., Muhaimin R. 2013. Analisis Mobilisasi Sel T CD4+ dan CD8+ pada Timus Ayam Pedaging Pasca Infeksi Salmonella typhimurium dan Pemberian Simplisia Polyscias obtusa. Jurusan Biologi FMIPA Universitas Brawijaya. Malang.
Pradana, A., Muhammad S. D., Muhaimin, R. . 2013. Mobilisasi CD4+, CD8+, dan B220+ pada Spleen Ayam Broiler dengan Pakan yang Mengandung Polyscias obtusa Pasca Infeksi Salmonella typhimurium. J.Exp. Life Sci. 2338-1655
Santos, R. L., Zhang S., Tsolis R. M., Kingsley R. A., Adams L. G., Baumler A. J. 2001. Animal Models of Salmonella Infections: Enteritis Versus Typhoid Fever. Microbes Infect. 3:1335–44.
Walton, R. E. 2008. Principle and Practice of Endodontic. Penerjemah: Surnawinata. EGC. Jakarta.
Cheeke, P. R. 2000. Actual And Potential Applications Of Yucca schidigera and Quillaja Saponaria Saponins In Human And Animal Nutrition. J Anim Sci 2000, 77:1-10.
Gondo, H. K. 2007. Penggunaan Antibiotika pada Kehamilan. Wijaya Kusuma, Vol I, No 1, Januari 2007, 57-62.
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