Activity of Dexamethasone Therapy on Pro-Inflammatory Cytokines Profile of Balb/c Mice with Biliary Atresia

Qonitatul Khasanah, Muhaimin Rifa'i

Abstract


ABSTRACT

Biliary atresia is a neonatal obstructive cholangiopathy that progresses to end-stage liver disease. Dexamethasone is one of synthetic glucocorticoid which has function as an anti-inflammatory agent. Here, we investigated whether dexamethasone could modulate the immune activity in mice strain Balb/c with biliary atresia based on the change of quantity of IFN-γ and TNF-α as a pro-inflammatory molecules. This study consists of 2 stages. The first stage is pre-condition which is made the biliary duct become fibrosis by injecting 20 µl of phosphate buffered saline containing 1.5 x 106 fluorescence-forming units Rhesus Rotavirus (RRV) subcutaneously on first day (24 hours) after the mice was born. The second stage is injection with dexamethasone with dose 0,5 mg/kg BW subcutaneously on the 7th-14th day and 14th-21st day. The clinical effect of dexamethasone is investigated on 14th and 21st day by flow cytometry method. Data were analyzed using Kruskal-Wallis test (p<0,05) and Mann-Whitney test using SPSS 16 for Windows. Rotavirus injection subcutaneously was proven to stimulate the production of proinflammatory cytokines, especially in the third week of termination. The result indicated an increasing number of proinflammatory cytokines such as IFN-γ and TNF-α after RRV injection but after injection of dexamethasone the number of those cytokines is decreased. It can be understood that dexamethasone has a capability to reduce the effect of inflammation regard to the decrease of proinflammatory cytokines.

Keywords : Biliary atresia, Dexamethasone, Inflammation, Obstructive, Rhesus Rotavirus


Full Text:

PDF

References


REFERENCES

Rifa’I, M., Y. Kawamoto, I. Nakashima, H. Suzuki. 2004. Essential roles of CD8+CD122+ regulatory T cells in the maintenance of T cell homeostasis. The Journal of experimental medicine. 200(9):1123-1124

de Carvalho E., Cláudia A. P I., Jorge A. B. 2007. Extrahepatic biliary atresia: current concepts and future directions. Jornal de Pediatria. 83(2):105-120

Moyer K, Kaimal V, Pacheco C. 2010. Staging of biliary atresia at diagnosis by molecular profiling of the liver. Genome Med; 2:33.

Sokol RJ, Mack C. 2001. Etiopathogenesis of biliary atresia. Semin Liver Dis; 21(4):517–24.

Lippi C, Chrousus GP. 1992. Glucocorticoids. In Yaffe SJ, Aranda JV eds.Pediatric Pharmacology, Theurapeutic Principles in Practice.WB Saunders, Philadelphia: 466 – 75

Jiang B., L. Snipes Magaldi,P. Dennehy,H. Keyserling. 2003. Cytokines as Mediators for or Effectors against Rotavirus Disease in Children. Clin Diag Lab Immunol; 10(6): 995-1001.

Elenkov IJ. 2004. Glucocorticoids and the Th1/Th2 balance. Ann N Y Acad Sci; 1024:138-46.

Abbas, A. K., dan Lichtman, A. H. 2005. Cellular and Molecular Immunology,Edisi Kelima. Elsevier. Philadelphia.

Dalhouise University. 2008. Cytokines. http://immunology.medicine.dal.ca/education/ Immunity.htm. Tanggal akses 11 November 2008.

Shivakumar P, Campbell KM, Sabla GE. 2004. Obstruction of extrahepatic bile ducts by lymphocytesis regulated by IFN-gamma in experimental biliary atresia. J. Clin. Invest; 114:322–9.

Detrick, B.,Nagineni, C.N., and J.J. Hooks. 2002. Transforming growth factor-beta in human retinal pigment epithelial cell is enhanched by Toxoplasma gondii : a possible role in the immunopathologenesis of retinochoroiditis. Clin. Exp. Immun. 128 (2): 372-378.

Katzung, Bertam G. 2002. Farmakologi Dasar dan Klinik Buku 2 Ed.8. Salemba Medika Glance. Jakarta.


Refbacks

  • There are currently no refbacks.