Analisa Flow Cytometry pada Subpopulasi Sel T-Limfosit Bursa Fabricius Ayam Pedaging Pasca Infeksi Salmonella typhimurium dan Pemberian Pakan Tambahan Polyscias obtusa

septi utami dewi, Mochammad Sasmito Djati, Muhaimin Rifa'i

Abstract


Tujuan penelitian  ini adalah mengetahui adanya aktivitas imunomodulator daun Polyscias obtusa pada organ bursa fabricius ayam  pedaging  yang terinfeksi bakteri Salmonella typhimurium, serta mengetahui dosis optimum penambahan daun Polyscias obtusa dalam mempengaruhi jumlah subpopulasi T-limfosit pada organ bursa fabricius ayam pedaging yang terinfeksi Salmonella typhimurium. Prosedur kerja yang dilakukan adalah konfirmasi isolat bakteri Salmonella typhimurium, pemberian daun Polyscias obtusa, infeksi bakteri Salmonella typhimurium, pembedahan, isolasi sel, analisis Flow Cytometri, analisis data. Hasil penelitian menunjukkan bahwa Pemberian pakan alami dengan tambahan daun Polyscias obtusa dengan variasi dosis 0,08%, 0,16%, tidak memberikan pengaruh terhadap jumlah relatif CD8+ dan B220+ pada Bursa fabricius. Sehingga, tidak ada perbedaan yang signifikan antara pakan pabrik dan pakan alami dengan tambahan daun Polyscias obtusa.. Namun, pada dosis 0,26 % fase finisher terlihat peningkatan jumlah relatif sel CD4+ yang siginifikan dibandingkan fase starter. Dapat disimpulkan bahwa, dosis 0,26% merupakan dosis optimum dalam meningkatkan proses proliferasi jumlah sel CD4+ sebagai agen imunomodulator pada bursa fabricius ayam pedaging.

 

Kata kunci :  Ayam pedaging, bursa fabricius, flow cytometry,   Polyscias obtusa, Salmonella typhimurium typhymurium


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References


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YH Lee, Y Ishida, M Rifa’i, Z Shi, K Isobe, H Suzuki . 2008. Essential role of CD8+CD122+ regulatory T cells in the recovery from experimental autoimmune encephalomyelitis. The Journal of Immunology 180 (2), 825-832

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Cutting edge: CD8+CD122+ regulatory T cells produce IL-10 to suppress IFN-gamma production and proliferation of CD8+ T cells.

SH Endharti AT, Rifa'I M, Shi Z, Fukuoka Y, Nakahara

Journal of immunology 175 (11), 7093-7097

Z Shi, Y Okuno, M Rifa'i, AT Endharti, K Akane, K Isobe, H Suzuki . 2009. Human CD8+CXCR3+ T cells have the same function as murine CD8+CD122+ Treg

European journal of immunology 39 (8), 2106-2119

Shi, Z, Rifa’i, M, YH Lee, H Shiku, K Isobe, H Suzuki. 2008. Importance of CD80/CD86–CD28 interactions in the recognition of target cells by CD8+CD122+ regulatory T cells. Immunology 124 (1), 121-128

Rifai'i, M. 2010. Andrographolide ameliorate rheumatoid arthritis by promoting the development of regulatory T cells. Journal of Tropical Life Science 1 (1), pp. 5-8

YH Lee, M Rifa'i. 2011. CD4+CD25+ FOXP3+ Regulatory T Cells In Allogeneic Hematopoietic Cell Transplantation. Journal of Tropical Life Science 1 (2), 69-75

M Rifa’i, Y Kawamoto, I Nakashima, H Suzuki . 2004. Essential roles of CD8+CD122+ regulatory T cells in the maintenance of T cell homeostasis. The Journal of experimental medicine 200 (9), 1123-1134

Endharti AT, Rifa'i M, Shi Z, Fukuoka Y, Nakahara Cutting edge: CD8+CD122+ regulatory T cells produce IL-10 to suppress IFN-gamma production and proliferation of CD8+ T cells. Journal of immunology 175 (11), 7093-7097

YH Lee, Y Ishida, M Rifa’i, Z Shi, K Isobe, H Suzuki . 2008. Essential role of CD8+CD122+ regulatory T cells in the recovery from experimental autoimmune encephalomyelitis. The Journal of Immunology 180 (2), 825-832

M Rifa’i, Z Shi, SY Zhang, YH Lee, H Shiku, K Isobe, H Suzuki . 2008. CD8+CD12+ regulatory T cells recognize activated T cells via conventional MHC class I–αβTCR interaction and become IL-10-producing active regulatory cells. International immunology 20 (7), 937-947

Z Shi, Y Okuno, M Rifa'i, AT Endharti, K Akane, K Isobe, H Suzuki . 2009. Human CD8+CXCR3+ T cells have the same function as murine CD8+CD122+ Treg

European journal of immunology 39 (8), 2106-2119

Z Shi, M Rifa’i, YH Lee, H Shiku, K Isobe, H Suzuki. 2008. Importance of CD80/CD86–CD28 interactions in the recognition of target cells by CD8+CD122+ regulatory T cells. Immunology 124 (1), 121-128

Rifai'i, M. 2010. Andrographolide ameliorate rheumatoid arthritis by promoting the development of regulatory T cells. Journal of Tropical Life Science 1 (1), pp. 5-8

YH Lee, M Rifa'i. 2011. CD4+CD25+ FOXP3+ Regulatory T Cells In Allogeneic Hematopoietic Cell Transplantation. Journal of Tropical Life Science 1 (2), 69-75


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