Uji Aktifitas Biologis Fraksi Ethanol Daun Sambiloto (Andrographis paniculata) Terhadap Perubahan Kuantitatif Sel T Regulator Pada Mencit BALB/c (Mus musculus)
Abstract
Andrografolid merupakan senyawa aktif sejenis diterpenoid yang terkandung dalam daun sambiloto dan berperan sebagai imunomodulator di dalam sistem imun. Penelitian ini bertujuan untuk mengetahui perubahan kuantitatif sel T regulator setelah pemberian fraksi ethanol daun sambiloto secara oral, mengetahui dosis optimum fraksi ethanol daun sambiloto dalam perubahan kuantitas sel T regulator, dan mengetahui histopatologis jaringan hepar setelah pemberian fraksi ethanol daun sambiloto. Metode meliputi ekstraksi daun sambiloto dengan pelarut ethanol 95%, perlakuan oral pada mencit BALB/c normal selama 2 minggu dengan dosis 0; 0,1; 0,5; dan 1 mg/g BB, isolasi sel limfosit organ spleen, analisis kuantitatif sel limfosit menggunakan haemocytometer dan flowcytometry, dan pembuatan preparat histologi hepar dengan pewarnaan HE. Analisis data menggunakan Rancangan Acak Lengkap (RAL) dengan uji ANOVA selang kepercayaan 95% menggunakan program SPSS 16 for Windows. Pemberian fraksi ethanol daun sambiloto secara oral meningkatkan jumlah sel T CD4+CD25+, pada Dosis 1 (0,1 mg/g BB) dan Dosis 2 (0,5 mg/g BB), selain itu terjadi penurunan jumlah sel T CD4+, CD4+CD62L+, dan CD8+CD62L+ pada Dosis 1 (0,1 mg/g BB) dan Dosis 3 (1 mg/g BB). Dosis optimum fraksi ethanol daun sambiloto dalam peningkatan sel T regulator adalah 0,1 mg/g BB. Fraksi ethanol daun sambiloto dosis 1 mg/g BB menimbulkan efek toksik yang signifikan pada jaringan hepar.
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Kata kunci: andrografolid, daun sambiloto, fraksi ethanol, histopatologi, imunomodulator
References
Sumaryono, W. 2002. Penelitian Obat Tradisional Indonesia dan Strategi Peningkatannya. Prosiding Seminar Nasional Tumbuhan Obat Indonesia XXI. Surabaya. hlm. 1–8.
Block, K.I. & M.N. Mead. 2003. Immune system effects of Echinacea, Ginseng and Astragalus: A review. Integrative cancer therapies. 2(3):247–267.
Tizard, I.R. 2000. Immunology: An Introduction. 6th Ed. Saunders College Publishing. New York.
Lee. J.H., H.H. Yu, L.C. Wang, Y.H. Yang, Y.T. Lin, & B.L. Chiang. 2006. The level of CD4+CD25+ Regualtory T Cells In Paediatric Patients With Allergic Rhinitis and Bronchial Asthma. Departement of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan, China and Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan, Cina.
Rifa’i, M. 2011. Alergi dan Hipersensitif. Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Braijaya, Malang.
Wahyuni, N. 2009. Perubahan Kadar Interleukin-2, Jumlah Relatif Sel T CD4+ dan CD8+, dan Histopatologi Usus Halus Mencit (BALB/c) Splenectomy Paparan Salmonella typhi. Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya.
Tedder, T.F., D.A. Steeber, A. Chen, & P. Engel. 1995. The Selectins: Vascular Adhesion Molecules. FASEB J. 9, 866–873.
Lesson. 1990. Buku Ajar Histologi. EGC Kedokteran. Jakarta
Mitaine AC, Marouf A, Haquei B, Bilirakis N, & Lacaille MA, 2001. Two Triterpenoid and Saponin from Achyrantes Bidentata. Chem. Pharm Bull. (Tokyo). 49(11):1492–1494.
El-Torki, M. A., A.W. Mehdi, A. I. Mohammed, N.A. Al-Jiboori. 2010. Liver and Kidney Functions in Adult Male Rats Following Multiple Subcutaneous Injections of Non-Lethal Doses of Datura metel Leaves Aqueous Extract. Zoology Departement, Faculty of Science, Garyounis University, Benghazi, Libya.
Rifai'i, M. 2010. Andrographolide ameliorate rheumatoid arthritis by promoting the development of regulatory T cells. Journal of Tropical Life Science 1 (1), pp. 5-8
YH Lee, M Rifa'i. 2011. CD4+CD25+ FOXP3+ Regulatory T Cells In Allogeneic Hematopoietic Cell Transplantation. Journal of Tropical Life Science 1 (2), 69-75
Shi, Z, Rifa’i, M, YH Lee, H Shiku, K Isobe, H Suzuki. 2008. Importance of CD80/CD86–CD28 interactions in the recognition of target cells by CD8+CD122+ regulatory T cells. Immunology 124 (1), 121-128
Z Shi, Y Okuno, M Rifa'i, AT Endharti, K Akane, K Isobe, H Suzuki . 2009. Human CD8+CXCR3+ T cells have the same function as murine CD8+CD122+ Treg
European journal of immunology 39 (8), 2106-2119
Endharti AT, Rifa'I M, Shi Z, Fukuoka Y, Nakahara Cutting edge: CD8+CD122+ regulatory T cells produce IL-10 to suppress IFN-gamma production and proliferation of CD8+ T cells.Journal of immunology 175 (11), 7093-7097
Rifa'i, M, Z Shi, SY Zhang, YH Lee, H Shiku, K Isobe, H Suzuki . 2008. CD8+CD12+ regulatory T cells recognize activated T cells via conventional MHC class I–αβTCR interaction and become IL-10-producing active regulatory cells. International immunology 20 (7), 937-947
YH Lee, Y Ishida, M Rifa’i, Z Shi, K Isobe, H Suzuki . 2008. Essential role of CD8+CD122+ regulatory T cells in the recovery from experimental autoimmune encephalomyelitis. The Journal of Immunology 180 (2), 825-832
Rifa'i, M, Y Kawamoto, I Nakashima, H Suzuki . 2004. Essential roles of CD8+CD122+ regulatory T cells in the maintenance of T cell homeostasis. The Journal of experimental medicine 200 (9), 1123-1134
M Rifa’i, Z Shi, SY Zhang, YH Lee, H Shiku, K Isobe, H Suzuki . 2008. CD8+CD12+ regulatory T cells recognize activated T cells via conventional MHC class I–αβTCR interaction and become IL-10-producing active regulatory cells. International immunology 20 (7), 937-947
YH Lee, M Rifa'i. 2011. CD4+CD25+ FOXP3+ Regulatory T Cells In Allogeneic Hematopoietic Cell Transplantation. Journal of Tropical Life Science 1 (2), 69-75
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