Pengaruh Hormon Hipofisa dan Ovaprim Terhadap Ovulasi Serta Pengaruh Pakan Terhadap Pertumbuhan Berudu Katak Fejervarya cancrivora
Abstract
Katak Fejervarya cancrivora merupakan kelompok dari kelas amfibi yang habitatnya sangat dipengaruhi oleh perubahan kondisi habitat dan aktivitas manusia. Salah satu upaya untuk mempertahankan keanekaragaman hayati yaitu memanfaatkan teknologi reproduksi buatan dengan melakukan induksi pematangan gonad menggunakan hormon hipofisa dan ovaprim. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian hormon hipofisa dan ovaprim terhadap pematangan gonad dan keberhasilan fertilisasi pada katak Fejervarya cancrivora, serta mengetahui pengaruh perbedaan pemberian pakan pada pertumbuhan berudu katak Fejervarya cancrivora. Injeksi dilakukan pada katak betina yang hampir matang gonad dengan total dosis injeksi 250 µl secara intraperitonial. Setelah dipijahkan dan difertilisasi, telur dipelihara sampai menetas dan diberikan dua perlakuan pakan, yaitu pakan bayam rebus (P1) serta pakan bayam rebus dan kuning telur rebus (P2). Hasil penelitian menunjukkan pada perlakuan injeksi hipofisa dihasilkan telur 632 butir sel telur dan berhasil menetas 65,67% dan perlakuan injeksi ovaprim sebanyak 108 butir sel telur dan dapat menetas 75,92%. Hasil perlakuan P1 dan P2 pada kedua jenis injeksi tidak terdapat perbedaan panjang (p>0,05) namun terdapat perbedaan berat (p<0,05) pada perlakuan selama masa metamorfosis. Persentase jumlah berudu yang berhasil bermetamorfosis sempurna pada perlakuan injeksi hipofisa sebanyak 41,12%, sedangkan pada perlakuan injeksi ovaprim sebanyak 31,67%.References
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